ARG63881
anti-14-3-3 sigma / Stratifin antibody
anti-14-3-3 sigma / Stratifin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes 14-3-3 sigma / Stratifin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 14-3-3 sigma / Stratifin |
| Antigen Species | Human |
| Immunogen | C-DLHTLSEDSYKDST |
| Conjugation | Un-conjugated |
| Alternate Names | YWHAS; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SFN |
| Gene Full Name | stratifin |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression. [UniProt] |
| Highlight | Related products: 1433 sigma antibodies; 1433 sigma Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Has “Obesity gene” been found? |
| Research Area | Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 28 kDa |
| PTM | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
Images (3) Click the Picture to Zoom In
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ARG63881 anti-14-3-3 sigma / Stratifin antibody WB image
Western blot: 30 µg of Mouse lung lysate stained with ARG63881 anti-14-3-3 sigma / Stratifin antibody at 1:1500 dilution.
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ARG63881 anti-14-3-3 sigma / Stratifin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skin tissue. Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63881 anti-14-3-3 sigma / Stratifin antibody at 2 µg/ml dilution followed by HRP-staining.
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ARG63881 anti-14-3-3 sigma / Stratifin antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Skin. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63881 anti-14-3-3 sigma / Stratifin antibody at 2.5 µg/ml dilution followed by AP-staining.
Customer's Feedback
Good
Review for anti-14-3-3 sigma / Stratifin antibody
Application：WB
Sample：Rat lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-14-3-3 sigma / Stratifin antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
