ARG41003
anti-14-3-3 alpha + beta antibody
anti-14-3-3 alpha + beta antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes 14-3-3 alpha + beta |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | This antibody might also react to other 14-3-3 proteins based on sequence alalysis: 14-3-3 protein zeta/delta (88%), 14-3-3 protein theta (82%), 14-3-3 protein gamma (75%), 14-3-3 protein eta (76%). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 14-3-3 alpha + beta |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-246 of Human 14-3-3 beta (NP_647539.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Protein 1054; 14-3-3 protein beta/alpha; Protein kinase C inhibitor protein 1; HS1; GW128; KCIP-1; YWHAA; HEL-S-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 28 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | YWHAB |
| Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta |
| Background | This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. [UniProt] |
| Cellular Localization | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| Calculated MW | 28 kDa |
| PTM | The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG41003 anti-14-3-3 alpha + beta antibody IHC-P image
Immunohistochemistry: Human tonsil stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:200 dilution.
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ARG41003 anti-14-3-3 alpha + beta antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:100 dilution.
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ARG41003 anti-14-3-3 alpha + beta antibody WB image
Western blot: Mouse Liver and Mouse Kidney stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:1000 dilution.
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ARG41003 anti-14-3-3 alpha + beta antibody WB image
Western blot: HepG2 stained with ARG41003 anti-14-3-3 alpha + beta antibody at 1:1000 dilution.
