ARG70218

SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter)

SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter) for SDS-PAGE

publication_link Publication11

Overview

Product Description E. coli expressed, His-tagged (C-ter) SARS-CoV-2 Spike recombinant protein (S1 Subunit)
Tested Application SDS-PAGE
Target Name SARS-CoV-2 Spike protein (S1 Subunit)
Species Virus
A.A. Sequence Val16 - Cys671
Expression System E. coli

Properties

Form Powder
Purity >98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

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Images (1) Click the Picture to Zoom In

  • ARG70218 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70218 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter).

Specific References

Cytokine-Induced iNOS in A549 Alveolar Epithelial Cells: A Potential Role in COVID-19 Lung Pathology

Functional assay / 

Amelia Barilli et al.
Biomedicines.,  (2023)

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Detoxified synthetic bacterial membrane vesicles as a vaccine platform against bacteria and SARS-CoV-2

Functional assay / 

Kyong-Su Park et al.
J Nanobiotechnology.,  (2023)

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Growth Arrest of Alveolar Cells in Response to Cytokines from Spike S1-Activated Macrophages: Role of IFN-γ

Functional assay / Human

Amelia Barilli et al.
Biomedicines,  (2022)

publication_link

 

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The JAK1/2 Inhibitor Baricitinib Mitigates the Spike-Induced Inflammatory Response of Immune and Endothelial Cells In Vitro

Amelia Barilli et al.
Biomedicines,  (2022)

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SARS-CoV-2 Spike protein is not pro-inflammatory in human primary macrophages: endotoxin contamination and lack of protein glycosylation as possible confounders

Functional Assay / Virus

Gloria Cinquegrani et al.
Cell Biol Toxicol.,  (2022)

publication_link

 

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Immune-Mediated Inflammatory Responses of Alveolar Epithelial Cells: Implications for COVID-19 Lung Pathology

Functional assay / 

Amelia Barilli et al.
Biomedicines .,  (2022)

publication_link

 

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In Vitro Exposure of Primary Human T Cells and Monocytes to Polyclonal Stimuli Reveals a Basal Susceptibility to Display an Impaired Cellular Immune Response and Develop Severe COVID-19

Cell Stimulation / 

Rebeca Viurcos-Sanabria et al.
Front Immunol .,  (2022)

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Standardized Extract of Asparagus officinalis Stem Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages

Functional assay / Virus

Ken Shirato et al.
Molecules,  (2021)

publication_link

 

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Endothelial Cell Activation by SARS-CoV-2 Spike S1 Protein: A Crosstalk between Endothelium and Innate Immune Cells

Stimulation  / 

Bianca Maria Rotoli et al.
Biomedicines.,  (2021)

publication_link

 

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ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages.

Ken Shirato et al.
Preprint.,  (2021)

publication_link

 

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SARS-CoV-2 spike protein S1 subunit induces pro-inflammatory responses via toll-like receptor 4 signaling in murine and human macrophages

Stimulation / 

Ken Shirato et al.
Heliyon .,  (2021)

publication_link

 

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