ARG81650
Rat sCD54 / sICAM1 ELISA Kit
Rat sCD54 / sICAM1 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81650-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81650-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81650-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81650-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81650-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81650-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81650-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81650-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81650-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81650-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81650-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81650 Rat sCD54 / sICAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat sCD54 / sICAM1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | sCD54 / sICAM1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3%; Inter-Assay CV: 7.0% |
| Alternate Names | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ICAM1 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Background | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. [UniProt] |
| Highlight | Related products: CD54 antibodies; CD54 ELISA Kits; CD54 Duos / Panels; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81650 Rat sCD54 / sICAM1 ELISA Kit standard curve image
ARG81650 Rat sCD54 / sICAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81650 Rat CD54 / ICAM1 ELISA Kit User's manual |
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