ARG80242
Rat VEGF ELISA Kit
Rat VEGF ELISA Kit for ELISA and Rat
Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80242-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80242-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80242-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80242-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80242-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80242-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80242-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80242-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80242-009 | TMB substrate | 12 ml | 4°C (Protect from light) |
| ARG80242-010 | STOP solution | 12 ml | 4°C |
| ARG80242-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80242 Rat VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat VEGF in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Mouse VEGF-B186 |
| Target Name | VEGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P16612 Rat Vascular endothelial growth factor A |
|---|---|
| Gene Symbol | Vegfa |
| Gene Full Name | vascular endothelial growth factor A |
| Background | This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Related news: Detecting MMPs and their non-ECM substrates The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
-
ARG80242 Rat VEGF ELISA Kit standard curve image
ARG80242 Rat VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80242 Rat VEGF ELISA Kit User's manual |
 Download
|
