ARG81880
Rat TIMP2 ELISA Kit
Rat TIMP2 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81880-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81880-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81880-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81880-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81880-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81880-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81880-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81880-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81880-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81880-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81880-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81880 Rat TIMP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat TIMP2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TIMP2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7%; Inter-Assay CV: 6.8% |
| Alternate Names | TIMP-2; Tissue inhibitor of metalloproteinases 2; CSC-21K; DDC8; Metalloproteinase inhibitor 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TIMP2 |
| Gene Full Name | TIMP metallopeptidase inhibitor 2 |
| Background | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008] |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. [UniProt] |
| Highlight | Related products: TIMP2 antibodies; TIMP2 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The activity of TIMP2 is dependent on the presence of disulfide bonds. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81880 Rat TIMP2 ELISA Kit standard curve image
ARG81880 Rat TIMP2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81880 Rat TIMP2 ELISA Kit User manual |
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