ARG82075
Rat MMP1 ELISA Kit
Rat MMP1 ELISA Kit for ELISA and Rat
Overview
| Product Description | ARG82075 Rat MMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat MMP1 in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | The following recombinant Rat proteins were tested and exhibited no cross-reactivity or interference: Adiponectin, ApoAI, BMP7, CCL2, CRP, FGF acidic, HGF, HSP27, IGF1, IL1 alpha, IFN alpha, IFN beta, IFN gamma, MMP2, MMP3, MMP9, PDGF, PLA2G7, prolactin, TLR4, TGF beta 1, TNF alpha, TNF RI, TNF RII, VEGF proteins. |
| Target Name | MMP1 |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 18 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 93 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5%; Inter-Assay CV: 10% |
| Alternate Names | MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | MMP1 |
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| Gene Full Name | matrix metallopeptidase 1 |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009] |
| Function | Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Highlight | Related products: MMP1 antibodies; MMP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82075 Rat MMP1 ELISA Kit standard curve image
ARG82075 Rat MMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82075 Rat MMP1 ELISA Kit User manual |
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