ARG80235
Rat IL1 beta ELISA Kit
Rat IL1 beta ELISA Kit for ELISA and Rat
Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80235-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80235-002 | Standard (Lyophilized) | 3 X 3.2 ng/vial | 4°C |
| ARG80235-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80235-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80235-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80235-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80235-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80235-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80235-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80235-010 | STOP solution | 12ml | 4°C |
| ARG80235-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80235 Rat IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL1 beta in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-2,4,1 alpha,sIL-2R,sIL-4R；mouse IL-2,4,10,1 alpha；rat GM-CSF, IL-2,4,10,1 alpha, SCF, TNF- alpha |
| Target Name | IL1 beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 30 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Il1b |
|---|---|
| Gene Full Name | interleukin 1 beta |
| Background | IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008] |
| Function | IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt] |
| Highlight | Related products: IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines Activation of IL-1 beta and IL-18 by Canonical Inflammasome Pathway Exploring Antiviral Immune Response RIP1 activation and pathogenesis of NASH New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit |
| PTM | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. |
Images (1) Click the Picture to Zoom In
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ARG80235 Rat IL1 beta ELISA Kit standard curve image
ARG80235 Rat IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80235 Rat IL1 beta ELISA Kit User's manual |
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