ARG81916

Rat CD44 ELISA Kit

Rat CD44 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81916-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81916-002 Standard 2 X 20 ng/vial 4°C
ARG81916-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81916-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81916-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81916-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81916-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81916-008 25X Wash buffer 20 ml 4°C
ARG81916-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81916-010 STOP solution 10 ml (Ready to use) 4°C
ARG81916-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81916 Rat CD44 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CD44 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD44
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.4%; Inter-Assay CV: 7.6%
Alternate Names MDU2; MDU3; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Extracellular matrix receptor III; PGP-I; Epican; CDW44; Phagocytic glycoprotein 1; Pgp1; HUTCH-I; MC56; Hyaluronate receptor; CD antigen CD44; Heparan sulfate proteoglycan; CD44 antigen; LHR; IN; HCELL; Phagocytic glycoprotein I; PGP-1; CSPG8; MIC4; ECMR-III; CDw44

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25406 Rat CD44

Swiss-port # P26051 Rat CD44 antigen

Gene Symbol CD44
Gene Full Name CD44 molecule (Indian blood group)
Background The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. [UniProt]
Highlight Related products:
CD44 antibodies; CD44 ELISA Kits; CD44 Duos / Panels;
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Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
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PTM Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.

N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.

Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81916 Rat CD44 ELISA Kit standard curve image

    ARG81916 Rat CD44 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81916 Rat CD44 ELISA Kit User's manual download icon Download