ARG80246
Rat CCL2 / MCP1 ELISA Kit
Rat CCL2 / MCP1 ELISA Kit for ELISA and Rat
Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80246-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80246-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80246-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80246-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80246-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80246-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80246-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80246-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80246-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80246-010 | STOP solution | 12ml | 4°C |
| ARG80246-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80246 Rat CCL2 / MCP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat CCL2 / MCP1 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Mouse CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, CCL6/C10, CCL7/MCP-3/MARC, CCL11/Eotaxin, CCL12/MCP-5, CXCL1/KC, CXCL2/MIP-2, G-CSF, GM-CSF, IFN- gamma, IL-1 alpha,1 beta,2,3,4,5,6；human CCL2/MCP-1, CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, CCL7/MCP-3, CCL8/MCP-2 |
| Target Name | CCL2 / MCP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Ccl2 |
| Gene Full Name | chemokine (C-C motif) ligand 2 |
| Background | This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Related news: HMGB1 in inflammation Inflammatory Cytokines Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit |
| PTM | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. |
Images (1) Click the Picture to Zoom In
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ARG80246 Rat CCL2 / MCP1 ELISA Kit standard curve image
ARG80246 Rat CCL2 / MCP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80246 Rat MCP-1 / CCL2 ELISA Kit User's manual |
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