ARG30330

Pyroptosis Antibody Panel

publication_link Publication17

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG57293 anti-Caspase 1 antibody Rabbit pAb Hu, Ms ICC/IF, IHC-P, IP, WB 20 μl
ARG41404 anti-GSDMD antibody Rabbit pAb Hu, Ms, Rat ICC/IF, WB 20 μl
ARG66285 anti-IL1 beta antibody [SQab1748] Mouse mAb Hu, Ms ELISA, FACS, ICC/IF, IHC-P, WB 20 μg
ARG62347 anti-beta Tubulin antibody [BT7R] Mouse mAb Hu, Ms, Rat, Chk, Mk, Rb Dot, ELISA, ICC/IF, WB 20 μg

Overview

Product Description Pyroptosis Antibody Panel is an all-in-one solution to make pyroptosis research easy and economic. This antibody panel comprises antibodies against pyroptosis key players Caspase-1, GSDMD, and IL1 beta. All three antibodies are good for detecting both pro- and cleaved proteins. Moreover, a loading control antibody against Tubulin is also included. This panel is the best solution for studying pyroptosis.
Target Name Pyroptosis
Alternate Names Pyroptosis antibody; GSDMD antibody; Caspase 1 antibody; beta Tubulin antibody; IL1 beta antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Panel for Pyroptosis
Highlight Related news:
Inflammasome & Pyroptosis Antibody Panels are released
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
AMPK signaling regulates NLRP3 inflammation and pyroptosis
Solutions for studying PANoptosis & PANoptosome

Images (16) Click the Picture to Zoom In

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: 20 μg of PC3, MCF7, MDA-MD-231, K562, M. brain, R. brain, MCF7, HT29, 3T3 and Raw264.7 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:3000 dilution.

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:10000 dilution.

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 10 µg of HeLa and 20 µg of Mouse brain lysates stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 20 μg of A431 and HeLa cell lysates stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:4000 dilution.

  • ARG57293 anti-Caspase 1 antibody WB image

    Western blot: 25 µg of cell lysate from PMA treated THP-1 cells were stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody ICC/IF image

    Immunofluorescence: Raw264.7 cells stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image

    Immunofluorescence: MCF7 cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:200 dilution.

  • ARG41404 anti-GSDMD antibody ICC/IF image

    Immunofluorescence: RAW264.7 cells stained with ARG41404 anti-GSDMD antibody at 1:100 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] FACS image

    Flow Cytometry: HeLa cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1 µg/ml (red) and without antibody control (black).

  • ARG57293 anti-Caspase 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG57293 anti-Caspase 1 antibody at 1:100 dilution.

  • ARG41404 anti-GSDMD antibody WB image

    Western blot: 25 µg of Mouse lung lysate stained with ARG41404 anti-GSDMD antibody at 1:1000 dilution.

  • ARG57293 anti-Caspase 1 antibody IP image

    Immunoprecipitation: 200 µg extracts of THP-1 cells Immunoprecipitated and stained with ARG57293 anti-Caspase 1 antibody at 1:1000 dilution.

  • ARG62347 anti-beta Tubulin antibody [BT7R] WB image

    Western blot: U87-MG and SW480 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:1000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image

    Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66285 anti-IL1 beta antibody [SQab1748] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 50 µg of Mouse liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.

  • ARG66285 anti-IL1 beta antibody [SQab1748] WB image

    Western blot: 50 µg of Rat liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.

Specific References

Endophilin A2 controls touch and mechanical allodynia via kinesin-mediated Piezo2 trafficking

ARG62347: WB / Mouse

Man-Xiu Xie et al.
Mil Med Res.,  (2024)

publication_link

 

hr_line

KDM3A Ablation Activates Endogenous Retrovirus Expression to Stimulate Antitumor Immunity in Gastric Cancer

ARG62347: WB / Human

Jiabin Zheng et al.
Adv Sci (Weinh).,  (2024)

publication_link

 

hr_line

Overexpression of EphB2 in the basolateral amygdala is crucial for inducing visceral pain sensitization in rats subjected to water avoidance stress

ARG62347: WB / Rat

Guang-Bing Duan et al.
CNS Neurosci Ther.,  (2024)

publication_link

 

hr_line

Apigenin Alleviated High-Fat-Diet-Induced Hepatic Pyroptosis by Mitophagy-ROS-CTSB-NLRP3 Pathway in Mice and AML12 Cells

ARG41404: WB / Mouse

Zhuoqun Meng et al.
J Agric Food Chem.,  (2023)

publication_link

 

hr_line

Ginsenoside Rb1 alleviated concanavalin A-induced hepatocyte pyroptosis by activating mitophagy

ARG66285: WB / Human, Mouse

Qian Zhang et al.
Food Funct.,  (2023)

publication_link

 

hr_line

Elaidic acid induced hepatocyte pyroptosis via autophagy-CTSB-NLRP3 pathway

ARG41404: WB / Mouse

Jing Lu et al.
Food Chem Toxicol.,  (2023)

publication_link

 

hr_line

STING activation in cardiomyocytes drives hypertrophy-associated heart failure via NF-κB-mediated inflammatory response

ARG62347: WB / Human, Mouse

Lintao Wang et al.
Biochim Biophys Acta Mol Basis Dis.,  (2023)

publication_link

 

hr_line

Ursolic acid induces apoptosis and pyroptosis in Reh cells by upregulating of the JNK signalling pathway based on network pharmacology and experimental validation

ARG57293: WB; ARG66285: WB / Human

Ying Luo et al.
Heliyon.,  (2023)

publication_link

 

hr_line

黄芪甲苷预处理对大鼠肠缺血再灌注所致肺损伤的影响及其机制

ARG57293: WB / Rat

王蕾 et al.
中国中西医结合外科杂志,  (2023)

publication_link

 

hr_line

Discrepant Activation Pattern of Inflammation and Pyroptosis Induced in Dermal Fibroblasts in Response to Dengue Virus Serotypes 1 and 2 and Nonstructural Protein 1

ARG57293: WB / Human

Kai-Che Wei et al.
Microbiol Spectr.,  (2023)

publication_link

 

hr_line

Protective effect of ginsenoside Rh2 against Toxoplasma gondii infection-induced neuronal injury through binding TgCDPK1 and NLRP3 to inhibit microglial NLRP3 inflammasome signaling pathway

ARG57293: WB / Mouse

Guang-Nan Jin et al.
Int Immunopharmacol.,  (2022)

publication_link

 

hr_line

Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2‑induced pyroptosis

ARG41404, ARG66285: WB; ARG57293:IF, WB / Human

Yulin Yuan et al.
Mol Med Rep,  (2022)

publication_link

 

hr_line

Curcumin improves the function of umbilical vein endothelial cells by inhibiting H2O2 induced pyroptosis

ARG57293:IF / Human

Yulin Yuan et al.
Molecular Medicine Reports,  (2022)

publication_link

 

hr_line

Curcumin Ameliorates White Matter Injury after Ischemic Stroke by Inhibiting Microglia/Macrophage Pyroptosis through NF- κ B Suppression and NLRP3 Inflammasome Inhibition

ARG41404: WB / Mouse

Yuanyuan Ran et al.
Oxid Med Cell Longev.,  (2021)

publication_link

 

hr_line

Aronia Melanocarpa Polysaccharide Ameliotates Inflammation and Aging in Mice by Modulating AMPK/SIRT1/NF-κB Signaling Pathway and Gut Microbiota

ARG41404: WB; ARG66285: WB; ARG57293: WB / Mouse

Yingchun Zhao et al.
Preprint.,  (2021)

publication_link

 

hr_line

Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps.

ARG66285: WB / Human

Yan Wang et al.
Mol Med Rep.,  (2021)

publication_link

 

hr_line

Abscisic acid ameliorates oxidative stress, inflammation, and apoptosis in thioacetamide-induced hepatic fibrosis by regulating the NF-кB signaling pathway in mice.

ARG66285: WB / Mouse

Xueyan Chen et al.
Eur J Pharmacol.,  (2020)

publication_link

 

hr_line