ARG30220
Phospho Raf1 Antibody Duo (Total, pS259)
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG51510 | anti-Raf1 phospho (Ser259) antibody | Rabbit pAb | Hu, Ms, Rat | IHC-P, WB | 50 μl |
| ARG10060 | anti-Raf1 antibody [410] | Mouse mAb | Hu | WB | 50 μg |
Overview
| Product Description | c-RAF, or RAF1 is a Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 can be deactivated through the phosphorylation by cyclic AMP-dependent protein Kinase (PKA) at various residues. Serine 43 phosphorylation decreased the binding to Ras in serum-starved cells. Mutation of Ser259 and Ser338 increased the basal Raf-1 activity. Dhillon et al. 2002. Mol Cell Biol 22(10):3237-46 Kubicek et al. 2002. J Biol Chem 277(10): 7913 |
|---|---|
| Target Name | Raf1 |
| Alternate Names | Phospho Raf1 antibody; Raf1 antibody; Raf1 phospho (Ser259) antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Phospho Raf1 Antibody Duo (Total, pS259) |
|---|---|
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
Images (2) Click the Picture to Zoom In
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ARG51510 anti-Raf1 phospho (Ser259) antibody WB image
Western Blot: extracts from 293 and MDA cells untreated or treated with PMA stained with anti-Raf1 (phospho Ser259) antibody ARG51510.
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ARG51510 anti-Raf1 phospho (Ser259) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-Raf1 (phospho Ser259) antibody ARG51510.
