ARG82989
Mouse TNF alpha ELISA Kit
Mouse TNF alpha ELISA Kit for ELISA and Mouse
Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82989-01 | Antibody Coated Microplate | 8 x 12 strips | 4°C |
| ARG82989-02 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG82989-03 | Standard / Sample Diluent Buffer | 20 ml | 4°C |
| ARG82989-04 | 100X Antibody Conjugate Concentrate | 1 vial | ≤ -20°C |
| ARG82989-05 | 1000X HRP-Streptavidin Solution | 1 vial | 4°C (protect from light) |
| ARG82989-06 | Antibody Diluent Buffer | 35 ml | 4°C |
| ARG82989-07 | 10X Wash Buffer | 50 ml | 4°C |
| ARG82989-08 | TMB Substrate | 12 ml | 4°C (protect from light) |
| ARG82989-09 | Stop Solution | 12 ml | 4°C |
| ARG82989-10 | Plate sealer | 4 adhesive strips | Room temperature |
Overview
| Product Description | ARG82989 Mouse TNF alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TNF alpha in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | TNF alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 50 µl |
| Alternate Names | Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF |
Application Instructions
| Assay Time | 4.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Tnf |
|---|---|
| Gene Full Name | tumor necrosis factor |
| Background | Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. |
| Highlight | Related products: TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines A new role of fat – factory of circulating miRNAs LKB1 deficiency in T cells promotes gut tumors New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit |
| PTM | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. |
Images (1) Click the Picture to Zoom In
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ARG82989 Mouse TNF alpha ELISA Kit standard curve image
ARG82989 Mouse TNF alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82989 Mouse TNF alpha ELISA Kit User manual |
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