ARG80206

Mouse TNF alpha ELISA Kit

Datasheet

Publication12

Ordering Information

Package

Price (USD)

96 wells

425.00

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Component

Cat No	Component Name	Package	Temp
ARG80206-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80206-002	Standard (Lyophilized)	3 X 1 ng/vial	4°C
ARG80206-003	Standard diluent buffer	20 ml	4°C
ARG80206-004	Antibody conjugate concentrate	400 µl	4°C
ARG80206-005	Antibody diluent buffer	16 ml	4°C
ARG80206-006	HRP-Streptavidin concentrate	400 µl	4°C (Protect from light)
ARG80206-007	HRP-Streptavidin diluent buffer	16 ml	4°C
ARG80206-008	20X Wash buffer	50 ml	4°C
ARG80206-009	TMB substrate	12ml	4°C (Protect from light)
ARG80206-010	STOP solution	12ml	4°C
ARG80206-011	Plate sealer	4 strips	Room temperature

Overview

Product Description	ARG80206 Mouse TNF alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TNF alpha in Serum, Plasma, Cell culture supernatants.
Tested Reactivity	Ms
Tested Application	ELISA
Specificity	No significant cross-reactivity or interference with Human Lymphotoxin alpha1/ beta2, Lymphotoxin alpha2/ beta1, TNF- alpha, TNF RI, TNF RII
Target Name	TNF alpha
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm
Sensitivity	15 pg/ml
Sample Type	Serum, Plasma, Cell culture supernatants
Standard Range	31.25 - 2000 pg/ml
Sample Volume	100 µl
Precision	CV: less than10%
Alternate Names	Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Assay Time	4 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 21926 Mouse TNF Swiss-port # P06804 Mouse Tumor necrosis factor
Gene Symbol	Tnf
Gene Full Name	tumor necrosis factor
Background	Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Highlight	Related products: TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines A new role of fat – factory of circulating miRNAs LKB1 deficiency in T cells promotes gut tumors New ELISA data calculation tool: Simplify the ELISA analysis by GainData
Research Area	Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit; Signaling Transduction kit
PTM	The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Images (1) Click the Picture to Zoom In

ARG80206 Mouse TNF alpha ELISA Kit standard curve image

ARG80206 Mouse TNF alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage

ELISA / Mouse / Tissue lysate

Bang-Chuan Hu et al.
Life Sci., (2023)

Macrophage-derived exosomal TNF-α promotes pulmonary surfactant protein expression in PM2.5-induced acute lung injury

ELISA / Mouse / Cell culture supernatant

Xiaoqi Hu et al.
Sci Total Environ., (2023)

Silencing IL12p35 Promotes Angiotensin II-Mediated Abdominal Aortic Aneurysm through Activating the STAT4 Pathway.

ELISA / Mouse / Serum

Lanlan Wang et al.
Mediators Inflamm., (2021)

Caulerpa lentillifera Polysaccharides-Rich Extract Reduces Oxidative Stress and Proinflammatory Cytokines Levels Associated with Male Reproductive Functions in Diabetic Mice.

ELISA / Mouse / Plasma

Khairiyah Khairuddin et al.
Appl. Sci., (2020)

Identification of N-Phenyl-7 H-pyrrolo[2,3- d]pyrimidin-4-amine Derivatives as Novel, Potent, and Selective NF-κB Inducing Kinase (NIK) Inhibitors for the Treatment of Psoriasis.

ELISA / Mouse / Serum

Zhu Yuqin et al.
J Med Chem., (2020)

Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes

ELISA / Mouse / Serum

Meiyan Wu et al.
J Adv Res., (2020)

Porphyromonas gingivalis induces depression via downregulating p75NTR-mediated BDNF maturation in astrocytes.

ELISA / Mouse / Tissue lysate

Wang Yi-Xi et al.
Brain Behav Immun., (2019)

In situ detecting changes in membrane lipid phenotypes of macrophages cultured in different cancer microenvironments using mass spectrometry.

ELISA / Mouse

Xu Yupin et al.
Analytica Chimica Acta, (2018)

Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice.

ELISA / Mouse

Sudirman S et al.
PLoS One., (2018)

AAV serotype 8-mediated liver specific GNMT expression delays progression of hepatocellular carcinoma and prevents carbon tetrachloride-induced liver damage.

ELISA / Mouse

Fang Cheng-Chieh et al.
Sci Rep., (2018)

Red Seaweed Eucheuma cottonii Ethanol Extract Regulates Inflammatory Mediators Production and Prevents Colonic Injury on Acute Colitis Disease in Mice

ELISA / Mouse

Sabri Sudirman et al.
Preprint., (2018)

Therapeutic effects of matrine derivate MASM in mice with collagen-induced arthritis and on fibroblast-like synoviocytes.

ELISA / Mouse

Yuming Zou et al.
Sci Rep. , (2017)