ARG81674
Mouse TIMP1 ELISA Kit
Mouse TIMP1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81674-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81674-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81674-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81674-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81674-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81674-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81674-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81674-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81674-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81674-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81674-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81674 Mouse TIMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse TIMP1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TIMP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7%; Inter-Assay CV: 7.2% |
| Alternate Names | Erythroid-potentiating activity; TIMP; Collagenase inhibitor; Fibroblast collagenase inhibitor; TIMP-1; EPO; CLGI; Tissue inhibitor of metalloproteinases 1; HCI; Metalloproteinase inhibitor 1; EPA |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TIMP1 |
| Gene Full Name | TIMP metallopeptidase inhibitor 1 |
| Background | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008] |
| Function | Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. [UniProt] |
| Highlight | Related products: TIMP1 antibodies; TIMP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81674 Mouse TIMP1 ELISA Kit standard curve image
ARG81674 Mouse TIMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81674 Mouse TIMP1 ELISA Kit User's manual |
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