ARG81295
Mouse/Rat Insulin (Ultra sensitive) ELISA Kit
Mouse/Rat Insulin (Ultra sensitive) ELISA Kit for ELISA and Mouse,Rat
Publication1
Overview
| Product Description | ARG81295 Mouse/Rat Insulin (Ultra sensitive) ELISA Kit is an ultra sensitive Enzyme Immunoassay kit for the quantification of Mouse/Rat Insulin in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Ms, Rat |
| Tested Application | ELISA |
| Specificity | Mouse / Rat Insulin: 100% Hamster Insulin: Detected Rat C-peptide, Rat pancreatic polypeptide, Porcine glucagon, Human Insulin like growth factor-I and II: Not Detected |
| Target Name | Insulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 50 pg/ml (5 µl sample volume); 5 pg/ml (100 µl sample volume) |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 0.1 - 6.4 ng/ml |
| Sample Volume | 5 µl or 100 µl |
| Alternate Names | IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN; Insulin-2; InsII; Ins-2 |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Ins2 |
| Gene Full Name | insulin II |
| Background | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt] |
| Highlight | Related products: Insulin antibodies; Insulin ELISA Kits; Insulin Duos / Panels; Insulin recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81295 Mouse Insulin (Ultra sensitive) ELISA Kit standard curve image
ARG81295 Mouse Insulin (Ultra sensitive) ELISA Kit results of a typical standard run with optical density reading at 450 - 630 nm.
Specific References
GLP-1/GLP-1R Signaling Regulates Ovarian PCOS-Associated Granulosa Cells Proliferation and Antiapoptosis by Modification of Forkhead Box Protein O1 Phosphorylation Sites.
ELISA / Mouse / Serum
| Title | Download Link |
|---|---|
| ARG81295 Mouse/Rat Insulin (Ultra sensitive) ELISA Kit User's manual |
 Download
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