ARG81756
Mouse RBP4 ELISA Kit
Mouse RBP4 ELISA Kit for ELISA and Mouse
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81756-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81756-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81756-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81756-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81756-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81756-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81756-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81756-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81756-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81756-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81756-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81756 Mouse RBP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse RBP4 in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | RBP4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 7.2% |
| Alternate Names | Plasma retinol-binding protein; MCOPCB10; 1-176; RDCCAS; RBP; Retinol-binding protein 4; PRBP; 1-182; 1-179; 1-181 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RBP4 |
| Gene Full Name | retinol binding protein 4, plasma |
| Background | This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008] |
| Function | Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. [UniProt] |
| Highlight | Related products: RBP4 antibodies; RBP4 ELISA Kits; RBP4 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81756 Mouse RBP4 ELISA Kit standard curve image
ARG81756 Mouse RBP4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
A novel mouse model of heart failure with preserved ejection fraction after chronic kidney disease induced by retinol through JAK/STAT pathway
ELISA / Mouse / Serum
| Title | Download Link |
|---|---|
| ARG81756 Mouse RBP4 ELISA Kit User's manual |
 Download
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