ARG81921

Mouse MMP8 ELISA Kit

Mouse MMP8 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81921-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81921-002 Standard 2 X 10 ng/vial 4°C
ARG81921-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81921-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81921-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81921-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81921-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81921-008 25X Wash buffer 20 ml 4°C
ARG81921-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81921-010 STOP solution 10 ml (Ready to use) 4°C
ARG81921-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81921 Mouse MMP8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MMP8 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MMP8
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.3%
Alternate Names HNC; PMNL collagenase; MMP-8; PMNL-CL; CLG1; Neutrophil collagenase; Matrix metalloproteinase-8; EC 3.4.24.34

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17394 Mouse MMP8

Swiss-port # O70138 Mouse Neutrophil collagenase

Gene Symbol MMP8
Gene Full Name matrix metallopeptidase 8
Background This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Function Can degrade fibrillar type I, II, and III collagens. [UniProt]
Highlight Related products:
MMP8 antibodies; MMP8 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG81921 Mouse MMP8 ELISA Kit standard curve image

    ARG81921 Mouse MMP8 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81921 Mouse MMP8 ELISA Kit User manual download icon Download