ARG81662
Mouse MMP3 ELISA Kit
Mouse MMP3 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81662-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81662-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81662-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81662-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81662-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81662-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81662-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81662-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81662-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81662-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81662-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81662 Mouse MMP3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MMP3 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is cross-reactivity with MMP10 approximately 2% and no detectable cross-reactivity with other MMPs. |
| Target Name | MMP3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4%; Inter-Assay CV: 6.6% |
| Alternate Names | Transin-1; CHDS6; EC 3.4.24.17; SL-1; STMY1; Matrix metalloproteinase-3; STR1; STMY; MMP-3; Stromelysin-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP3 |
| Gene Full Name | matrix metallopeptidase 3 |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| Function | Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. [UniProt] |
| Highlight | Related products: MMP3 antibodies; MMP3 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81662 Mouse MMP3 ELISA Kit standard curve image
ARG81662 Mouse MMP3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81662 Mouse MMP3 ELISA Kit User manual |
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