ARG81742
Mouse IL1RA ELISA Kit
Mouse IL1RA ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81742-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81742-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81742-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81742-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81742-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81742-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81742-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81742-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81742-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81742-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81742-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81742 Mouse IL1RA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL1RA in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL1RA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3%; Inter-Assay CV: 6.7% |
| Alternate Names | IL1 inhibitor; Interleukin-1 receptor antagonist protein; ICIL-1RA; MVCD4; IL1RA; DIRA; IL-1RN; Anakinra; IL1F3; IRAP; IL-1ra; IL-1ra3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25085 Mouse Interleukin-1 receptor antagonist protein |
|---|---|
| Gene Symbol | IL1RN |
| Gene Full Name | interleukin 1 receptor antagonist |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. [UniProt] |
| Highlight | Related products: IL1RA antibodies; IL1RA ELISA Kits; IL1RA recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81742 Mouse IL1RA ELISA Kit standard curve image
ARG81742 Mouse IL1RA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81742 Mouse IL1RA ELISA Kit User's manual |
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