ARG80196

Mouse IL1 beta ELISA Kit

Mouse IL1 beta ELISA Kit for ELISA and Mouse

Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
publication_link Publication10

Component

Cat No Component Name Package
ARG80196-001 Antibody-coated microplate 8 X 12 strips 4°C
ARG80196-002 Standard (Lyophilized) 3 vials 4°C
ARG80196-003 10X Antibody Conjugate 1.2 ml -20°C
ARG80196-004 40X HRP-Streptavidin solution 300 µl 4°C
ARG80196-005 Standard/Sample Diluent Buffer 20 ml 4°C
ARG80196-006 Antibody Diluent Buffer 35 ml 4°C
ARG80196-007 10X Wash buffer 50 ml 4°C
ARG80196-008 TMB substrate 12ml 4°C (Protect from light)
ARG80196-009 STOP solution 12ml 4°C
ARG80196-010 Plate sealer 3 adhesive strips

Overview

Product Description ARG80196 Mouse IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL1 beta in Serum, Plasma, Cell culture supernatants and tissue lysate.
Tested Reactivity Ms
Tested Application ELISA
Specificity No significant cross-reactivity or interference with mouse IL-1 alpha, IL-1ra, IL-1 RI/Fc Chimera, IL-1 RII/Fc Chimera
Target Name IL1 beta
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants and tissue lysate
Standard Range 15.6 - 1000 pg/ml
Sample Volume 50 µl
Precision CV: less than10%
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16176 Mouse IL1B

Swiss-port # P10749 Mouse Interleukin-1 beta

Gene Symbol Il1b
Gene Full Name interleukin 1 beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
Highlight Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins;
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New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Images (1) Click the Picture to Zoom In

  • ARG80196 Mouse IL1 beta ELISA Kit standard curve image

    ARG80196 Mouse IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Macrophage-derived exosomal TNF-α promotes pulmonary surfactant protein expression in PM2.5-induced acute lung injury

ELISA / Mouse / Cell culture supernatant

Xiaoqi Hu et al.
Sci Total Environ.,  (2023)

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Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage

ELISA / Mouse / Tissue lysate

Bang-Chuan Hu et al.
Life Sci.,  (2023)

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Macrophage secretory IL-1β promotes docetaxel resistance in head and neck squamous carcinoma via SOD2/CAT-ICAM1 signaling

ELISA / Mouse / Tissue lysate

Ching-Yun Hsieh et al.
JCI Insight,  (2022)

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Silencing IL12p35 Promotes Angiotensin II-Mediated Abdominal Aortic Aneurysm through Activating the STAT4 Pathway.

ELISA / Mouse / Serum

Lanlan Wang et al.
Mediators Inflamm.,  (2021)

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Caulerpa lentillifera Polysaccharides-Rich Extract Reduces Oxidative Stress and Proinflammatory Cytokines Levels Associated with Male Reproductive Functions in Diabetic Mice.

ELISA / Mouse / Plasma

Khairiyah Khairuddin et al.
Appl. Sci.,  (2020)

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Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice.

ELISA / Mouse

Sudirman S et al.
PLoS One.,  (2018)

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Red Seaweed Eucheuma cottonii Ethanol Extract Regulates Inflammatory Mediators Production and Prevents Colonic Injury on Acute Colitis Disease in Mice

ELISA / Mouse

Sabri Sudirman et al.
Preprint.,  (2018)

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Influences of Surface Coating of PLGA Nanoparticles on Immune Activation of Macrophages.

ELISA / Mouse

Chen Xinyi et al.
J. Mater. Chem. B,  (2018)

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Influences of size and surface coating of gold nanoparticles on inflammatory activation of macrophages.

ELISA / Mouse

Chen Xinyi et al.
Colloids Surf B Biointerfaces.,  (2017)

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Therapeutic effects of matrine derivate MASM in mice with collagen-induced arthritis and on fibroblast-like synoviocytes.

ELISA / Mouse

Yuming Zou et al.
Sci Rep. ,  (2017)

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