ARG80196
Mouse IL1 beta ELISA Kit
Mouse IL1 beta ELISA Kit for ELISA and Mouse
Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
Publication10
Component
| Cat No | Component Name | Package | |
|---|---|---|---|
| ARG80196-001 | Antibody-coated microplate | 8 X 12 strips | 4°C |
| ARG80196-002 | Standard (Lyophilized) | 3 vials | 4°C |
| ARG80196-003 | 10X Antibody Conjugate Mixture | 1.2 ml | -20°C |
| ARG80196-004 | 40X HRP-Streptavidin solution | 300 µl | 4°C |
| ARG80196-005 | Standard/Sample Diluent Buffer | 20 ml | 4°C |
| ARG80196-006 | Antibody Diluent Buffer | 35 ml | 4°C |
| ARG80196-007 | 10X Wash buffer | 35 ml | 4°C |
| ARG80196-008 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80196-009 | STOP solution | 12ml | 4°C |
| ARG80196-010 | Plate sealer | 4 adhesive strips |
Overview
| Product Description | ARG80196 Mouse IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL1 beta in Serum, Plasma, Cell culture supernatants and tissue lysate. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with mouse IL-1 alpha, IL-1ra, IL-1 RI/Fc Chimera, IL-1 RII/Fc Chimera |
| Target Name | IL1 beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants and tissue lysate |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Il1b |
| Gene Full Name | interleukin 1 beta |
| Background | IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008] |
| Function | IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt] |
| Highlight | Related products: IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines Activation of IL-1 beta and IL-18 by Canonical Inflammasome Pathway LKB1 deficiency in T cells promotes gut tumors Exploring Antiviral Immune Response RIP1 activation and pathogenesis of NASH New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit |
| PTM | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. |
Images (1) Click the Picture to Zoom In
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ARG80196 Mouse IL1 beta ELISA Kit standard curve image
ARG80196 Mouse IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage
ELISA / Mouse / Tissue homogenate
Macrophage-derived exosomal TNF-α promotes pulmonary surfactant protein expression in PM2.5-induced acute lung injury
ELISA / Mouse / Cell culture supernatant
Macrophage secretory IL-1β promotes docetaxel resistance in head and neck squamous carcinoma via SOD2/CAT-ICAM1 signaling
ELISA / Mouse / Tissue lysate
Silencing IL12p35 Promotes Angiotensin II-Mediated Abdominal Aortic Aneurysm through Activating the STAT4 Pathway.
ELISA / Mouse / Serum
Caulerpa lentillifera Polysaccharides-Rich Extract Reduces Oxidative Stress and Proinflammatory Cytokines Levels Associated with Male Reproductive Functions in Diabetic Mice.
ELISA / Mouse / Plasma
Influences of Surface Coating of PLGA Nanoparticles on Immune Activation of Macrophages.
ELISA / Mouse
Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice.
ELISA / Mouse
Red Seaweed Eucheuma cottonii Ethanol Extract Regulates Inflammatory Mediators Production and Prevents Colonic Injury on Acute Colitis Disease in Mice
ELISA / Mouse
Influences of size and surface coating of gold nanoparticles on inflammatory activation of macrophages.
ELISA / Mouse
Therapeutic effects of matrine derivate MASM in mice with collagen-induced arthritis and on fibroblast-like synoviocytes.
ELISA / Mouse
| Title | Download Link |
|---|---|
| ARG80196 Mouse IL1 beta ELISA Kit User's manual |
 Download
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