ARG82825
Mouse IGFBP2 ELISA Kit
Mouse IGFBP2 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82825-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82825-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82825-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82825-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82825-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82825-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82825-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82825-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82825-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82825-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82825-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82825 Mouse IGFBP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IGFBP2 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | IGFBP2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 7.2% |
| Alternate Names | IBP-2; IBP2; Insulin-like growth factor-binding protein 2; IGFBP-2; IGF-BP53; IGF-binding protein 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P47877 Mouse Insulin-like growth factor-binding protein 2 |
|---|---|
| Gene Symbol | IGFBP2 |
| Gene Full Name | insulin-like growth factor binding protein 2, 36kDa |
| Background | The protein encoded by this gene is one of six similar proteins that bind insulin-like growth factors I and II (IGF-I and IGF-II). The encoded protein can be secreted into the bloodstream, where it binds IGF-I and IGF-II with high affinity, or it can remain intracellular, interacting with many different ligands. High expression levels of this protein promote the growth of several types of tumors and may be predictive of the chances of recovery of the patient. Several transcript variants, one encoding a secreted isoform and the others encoding nonsecreted isoforms, have been found for this gene. [provided by RefSeq, Sep 2015] |
| Function | Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82825 Mouse IGFBP2 ELISA Kit standard curve image
ARG82825 Mouse IGFBP2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82825 Mouse IGFBP2 ELISA Kit User manual |
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