ARG81653

Mouse IGFBP1 ELISA Kit

Mouse IGFBP1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81653-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81653-002 Standard 2 X 10 ng/vial 4°C
ARG81653-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81653-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81653-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81653-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81653-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81653-008 25X Wash buffer 20 ml 4°C
ARG81653-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81653-010 STOP solution 10 ml (Ready to use) 4°C
ARG81653-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81653 Mouse IGFBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IGFBP1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IGFBP1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%; Inter-Assay CV: 6.9%
Alternate Names IBP-1; IBP1; PP12; IGF-BP25; Insulin-like growth factor-binding protein 1; hIGFBP-1; IGFBP-1; Placental protein 12; AFBP; IGF-binding protein 1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16006 Mouse IGFBP1

Swiss-port # P47876 Mouse Insulin-like growth factor-binding protein 1

Gene Symbol IGFBP1
Gene Full Name insulin-like growth factor binding protein 1
Background This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. [UniProt]
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PTM Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81653 Mouse IGFBP1 ELISA Kit standard curve image

    ARG81653 Mouse IGFBP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81653 Mouse IGFBP1 ELISA Kit User's manual download icon Download