ARG81818
Mouse CCL8 / MCP2 ELISA Kit
Mouse CCL8 / MCP2 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81818-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81818-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81818-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81818-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81818-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81818-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81818-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81818-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81818-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81818-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81818-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81818 Mouse CCL8 / MCP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CCL8 / MCP2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CCL8 / MCP2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0%; Inter-Assay CV: 6.4% |
| Alternate Names | SCYA10; MCP2; Small-inducible cytokine A8; 6-76; Monocyte chemotactic protein 2; HC14; SCYA8; C-C motif chemokine 8; MCP-2; Monocyte chemoattractant protein 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL8 |
| Gene Full Name | chemokine (C-C motif) ligand 8 |
| Background | This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014] |
| Function | Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. [UniProt] |
| Highlight | Related products: CCL8 antibodies; CCL8 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81818 Mouse CCL8 / MCP2 ELISA Kit standard curve image
ARG81818 Mouse CCL8 / MCP2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81818 Mouse CCL8 / MCP2 ELISA Kit User's manual |
 Download
|
