ARG81875
Mouse Angiopoietin 1 ELISA Kit
Mouse Angiopoietin 1 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81875-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81875-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81875-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81875-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81875-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81875-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81875-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81875-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81875-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81875-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81875-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81875 Mouse Angiopoietin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Angiopoietin 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Angiopoietin 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1%; Inter-Assay CV: 7.0% |
| Alternate Names | AGP1; Angiopoietin-1; ANG-1; AGPT; ANG1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANGPT1 |
| Gene Full Name | angiopoietin 1 |
| Background | This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015] |
| Function | Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. [UniProt] |
| Highlight | Related products: Angiopoietin antibodies; Angiopoietin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81875 Mouse Angiopoietin 1 ELISA Kit standard curve image
ARG81875 Mouse Angiopoietin 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81875 Mouse Angiopoietin 1 ELISA Kit User manual |
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