ARG30327

Microvascular Density Antibody Panel

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Publication45

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1 kit

399.00

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Datasheet
Publication (45)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG66347	anti-Ki-67 antibody [SQab1897]	Rabbit mAb	Hu	IHC-P	20 μl
ARG52748	anti-CD31 antibody	Rabbit pAb	Hu, Ms, Rat, Pig	ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB	20 μl
ARG66200	anti-Desmin antibody [SQab1722]	Rabbit mAb	Hu, Ms, Rat	IHC-P, WB	20 μl
ARG66381	anti-alpha smooth muscle Actin antibody [SQab18108]	Rabbit mAb	Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh	FACS, ICC/IF, IHC-Fr, IHC-P, WB	20 μl
ARG65351	Goat anti-Rabbit IgG antibody (HRP)	Goat pAb	Rb	ELISA, IHC-P, WB	50 μl

Overview

Product Description	Microvascular Density Antibody (MVD) Panel is an all-in-one solution to make angiogenesis research easy and economic. This antibody panel comprises the proliferation marker Ki-67 antibody, endothelial marker CD31 antibody, and mural cell markers Desmin and alpha-SMA antibodies. All the antibodies in this panel have excellent IHC performance. It is the best solution to examine the process of angiogenesis in tumor tissues and xenograft model. Related news: Angiogenesis & MVD Antibody Panels are released
Target Name	Microvascular Density
Alternate Names	Microvascular Density antibody; CD31 antibody; Desmin antibody; Ki-67 antibody; alpha smooth muscle Actin antibody

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name	Antibody Panel for Microvascular Density
Research Area	Angiogenesis antibody; MVD Antibody; Microvascular Density Antibody

Images (22) Click the Picture to Zoom In

ARG52748 anti-CD31 antibody IHC-P image

Immunohistochemistry: Formalin-fixed and Paraffin-embedded MDA-MB-231 (tumor xenograft study) stained with ARG52748 anti-CD31 antibody.
Antigen Retrieval Buffer: Bond Epitope Retrieval Solution.
ARG66200 anti-Desmin antibody [SQab1722] WB image

Western blot: 30 µg of MEF (negative control) and Mouse heart lysates stained with ARG66200 anti-Desmin antibody [SQab1722] at 1:5000 dilution.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image

Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.

From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.
ARG66200 anti-Desmin antibody [SQab1722] IHC-P image

Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human colon tissue stained with ARG66200 anti-Desmin antibody [SQab1722] at 1:8000 dilution.

Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
ARG66347 anti-Ki-67 antibody [SQab1897] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG66347 anti-Ki-67 antibody [SQab1897]. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0).
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
ARG52748 anti-CD31 antibody WB image

Western blot: 30 µg of HUVEC lysate stained with ARG52748 anti-CD31 antibody at 1:500 dilution.
ARG66200 anti-Desmin antibody [SQab1722] IHC-P image

Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human placenta tissue stained with ARG66200 anti-Desmin antibody [SQab1722] at 1:8000 dilution.

Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
ARG66347 anti-Ki-67 antibody [SQab1897] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human esophageal cancer tissue stained with ARG66347 anti-Ki-67 antibody [SQab1897]. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0).
ARG66347 anti-Ki-67 antibody [SQab1897] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cervical cancer tissue stained with ARG66347 anti-Ki-67 antibody [SQab1897]. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0).
ARG66347 anti-Ki-67 antibody [SQab1897] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human diffuse large B-cell lymphoma stained with ARG66347 anti-Ki-67 antibody [SQab1897]. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0).
ARG66347 anti-Ki-67 antibody [SQab1897] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human esophageal cancer tissue stained with ARG66347 anti-Ki-67 antibody [SQab1897]. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0).
ARG66347 anti-Ki-67 antibody [SQab1897] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil tissue stained with ARG66347 anti-Ki-67 antibody [SQab1897]. Antigen Retrieval: Heat mediation was performed using Tris/EDTA buffer (pH 9.0).
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.
ARG52748 anti-CD31 antibody IHC-P image

WB analysis of LLC xenograft mouse tumor tissue lysate stained with ARG52748 anti-CD31 antibody.

Reproduced under the Creative Commons license from Xu Fei et al. J Exp Clin Cancer Res., (2018) 37:207 Doi: 10.1186/s13046-018-0878-0, Fig. 7C.
ARG52748 anti-CD31 antibody WB image

WB analysis of LLC xenograft mouse tumor tissue lysate stained with ARG52748 anti-CD31 antibody.

Reproduced under the Creative Commons license from Xu Fei et al. J Exp Clin Cancer Res., (2018) 37:207 Doi: 10.1186/s13046-018-0878-0, Fig. 7C.
ARG52748 anti-CD31 antibody IHC image

Cryostat sections from Mouse Retinal stained with ARG52748 anti-CD31 antibody.

Reproduced under the Creative Commons license from Lin Chin-I et al. July/August 2019, 6(4) ENEURO.0391-18.2019 1–17, Doi: 10.1523/ENEURO.0391-18.2019, Fig. 4 A-C.

Specific References

Expression of elastin, FBXW2, fibrillin-1, and α-smooth muscle actin in damaged blood vessels of bovine legs

ARG66381: IHC-P / Bovine

Mari Akiyama et al.
Preprint, (2024)

Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction

ARG66381: WB / Mouse

Lei Qian et al.
Biomed Pharmacother., (2024)

Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice

ARG66381: IHC-P / Mouse

Zhenyu Feng et al.
Heliyon, (2024)

PCSK9 inhibitors ameliorate arterial stiffness in ACS patients: evidences from mendelian randomization, cohort studies and basic experiments

ARG66381: WB / Mouse

Linghao Xu et al.
Preprint, (2024)

Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation

ARG66381: IHC-P / Mouse

Qiu-Yue Lin et al.
BBA - Molecular Basis of Disease, (2024)

CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization

Mouse / ARG66381: IHC-P

Yun-Long Zhang et al.
J Adv Res., (2023)

Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis

ARG66381: IHC-P / Mouse

Jing Chang et al.
Front Pharmacol., (2023)

circ-hnRNPU inhibits NONO-mediated c-Myc transactivation and mRNA stabilization essential for glycosylation and cancer progression

ARG52748: IHC-Fr / Human

Hongjun Li et al.
J Exp Clin Cancer Res., (2023)

Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

ARG66381: IFC-Fr / Mouse

Masahiro Terasawa et al.
Cells., (2023)

Elastic Fibers and F-Box and WD-40 Domain-Containing Protein 2 in Bovine Periosteum and Blood Vessels

Cattle / ARG66381: IHC-P

Mari Akiyama et al.
Biomimetics (Basel)., (2023)

Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
J Cancer Res Clin Oncol ., (2022)

CT perfusion imaging can detect residual lung tumor early after radiofrequency ablation: a preliminary animal study on both tumoral and peri-tumoral region assessment

ARG52748: IHC-P / Rabbit

Zenghui Cheng et al.
J Thorac Dis., (2022)

Occludin facilitates tumour angiogenesis in bladder cancer by regulating IL8/STAT3 through STAT4

ARG52748: IHC-P / Human

Fan Yang et al.
J Cell Mol Med., (2022)

Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration

ARG66381: IHC-P / Mouse

Ze-Yang Qiu et al.
Front Pharmacol, (2022)

Inhibition of Angiogenesis and Tumor Progression of Mk-0429, an Integrin Αvβ3 Antagonist, on Oral Squamous Cell Carcinoma

ARG66381: IHC-P / Human

Takayuki Nakagawa et al.
Research Square, (2022)

Partial Reconstruction of Uterus Cervix in Rat by Decellularized Human Uterine Cervical Scaffold Combined with Adipose-Derived Stem Cells (ADSCs)

ARG52748: IHC-P / Mouse

Yuqi Li et al.
J Immunol Res, (2022)

Small Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhanced Proangiogenic Potential of Cardiac Fibroblasts via Angiopoietin-Like 4

ARG52748: WB / Human

Jiejie Li et al.
Stem Cells Int., (2022)

"Two-birds-one-stone" colon-targeted nanomedicine treats ulcerative colitis via remodeling immune microenvironment and anti-fibrosis

ARG66381: WB / Mouse

Jiaxin Zhang et al.
J Nanobiotechnology, (2022)

Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy

ARG52748: IHC-P / Mouse

Lili Liu et al.
Mater Today Bio., (2022)

ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis

ARG66381: ICC/IF / Human

Pengfei Zhu et al.
Pharmaceuticals (Basel)., (2022)

VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

ARG66381, ARG52748: IHC-P / Mouse

Qiu-Yue Lin et al.
Clin Transl Med., (2021)

Exploring the mechanism of Bushenhuoxue formula acting on postmenopausal osteoporosis via network pharmacology and experimental validation.

ARG52748: IHC-P / Mouse

Chunhai Ke et al.
Preprint., (2021)

G6PD-NF-κB-HGF Signal in Gastric Cancer-Associated Mesenchymal Stem Cells Promotes the Proliferation and Metastasis of Gastric Cancer Cells by Upregulating the Expression of HK2

ARG52748: IHC-P / Mouse

Bin Chen et al.
Front Oncol, (2021)

Heterogeneous microenvironmental stiffness regulates pro-metastatic functions of breast cancer cells

ARG52748: IHC-P / Mouse

Chun Liu et al.
Acta Biomater., (2021)

pH and Glucose Dual-Responsive Injectable Hydrogels with Insulin and Fibroblasts as Bioactive Dressings for Diabetic Wound Healing

ARG52748: IHC-P / Rat

Lingling Zhao et al.
ACS Appl Mater Interfaces., (2021)

Additive Antiproliferative and Antiangiogenic Effects of Metformin and Pemetrexed in a Non-Small-Cell Lung Cancer Xenograft Model.

ARG52748: IHC-P / Mouse

Jiun-Long Wang et al.
Front Cell Dev Biol., (2021)

Bushenhuoxue formula accelerates fracture healing via upregulation of TGF-β/Smad2 signaling in mesenchymal progenitor cells.

ARG52748: IHC-P / Mouse

Hu Songfeng et al.
Phytomedicine., (2020)

NIR-cleavable drug adducts of gold nanostars for overcoming multidrug-resistant tumors.

ARG52748: IHC-Fr / Mouse

Andrea C Del Valle et al.
Biomater Sci., (2020)

Amygdalin Promotes Fracture Healing through TGF- β/Smad Signaling in Mesenchymal Stem Cells.

ARG52748: IHC-P / Mouse

Jun Ying et al.
Stem Cells Int., (2020)

Platelet-rich plasma promotes bone formation, restrains adipogenesis and accelerates vascularization to relieve steroids-induced osteonecrosis of the femoral head.

ARG52748: IHC-P / Rat

Hui-Hui Xu et al.
Platelets., (2020)

Polydopamine-coated gold nanostar for combined antitumor and antiangiogenic therapy in multidrug-resistant breast cancer.

ARG52748: IHC-Fr / Mouse

You You-Hong et al.
Nanotheranostics., (2019)

Enhancing Microcirculation on Multitriggering Manner Facilitates Angiogenesis and Collagen Deposition on Wound Healing by Photoreleased NO from Hemin-Derivatized Colloids.

ARG52748: IHC-P / Mouse

Su Chia-Hao et al.
ACS Nano., (2019)

Peripheral Blood Stem Cell Therapy Does Not Improve Outcomes of Femoral Head Osteonecrosis With Cap-Shaped Separated Cartilage Defect.

ARG52748: IHC-P / Human

Ying Jun et al.
J Orthop Res., (2019)

Therapeutic targeting of circ-CUX1/EWSR1/MAZ axis inhibits glycolysis and neuroblastoma progression.

ARG52748: IHC-P / Human

Huanhuan Li et al.
EMBO Mol Med., (2019)

N-acetyl cysteine-loaded graphene oxide-collagen hybrid membrane for scarless wound healing.

ARG52748: IHC-P / Rat

Li Jialun et al.
Theranostics., (2019)

Blue Light Promotes Neurite Outgrowth of Retinal Explants in Postnatal ChR2 Mice.

ARG52748: IHC-Fr / Mouse

Lin Chin-I et al.
eNeuro., (2019)

Cis-acting circ-CTNNB1 promotes β-catenin signaling and cancer progression via DDX3-mediated transactivation of YY1.

ARG52748: IHC-P / Mouse

Yang Feng et al.
Cancer Res., (2018)

Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes.

ARG52748: IHC-P / Mouse

Chen Yajun et al.
Cell Death Differ., (2018)

Astragaloside IV inhibits lung cancer progression and metastasis by modulating macrophage polarization through AMPK signaling

ARG52748: IHC-P / Mouse

Xu Fei et al.
J Exp Clin Cancer Res., (2018)

Stiffness Modification of Photopolymerizable Gelatin-Methacrylate Hydrogels Influences Endothelial Differentiation of Human Mesenchymal Stem Cells.

ARG52748: ICC/IF / Human

Lin Chih-Hsin et al.
J Tissue Eng Regen Med., (2018)

Injectable bioactive akermanite/alginate composite hydrogels for in situ skin tissue engineering

ARG52748: IHC-Fr / Mouse

Han Y et al.
J. Mater. Chem. B., (2017)

Administration of erythropoietin prevents bone loss in osteonecrosis of the femoral head in mice

ARG52748: IHC-P / Mouse

Taotao Xu et al.
Mol Med Rep., (2017)

Multifunctional Hydrogels Prepared by Dual Ion Cross-Linking for Chronic Wound Healing.

ARG52748: IHC-P / Mouse

Li Y et al.
ACS Appl Mater Interfaces., (2017)

CO2 Delivery to Accelerate Incisional Wound Healing Following Single Irradiation of Near-Infrared Lamp on the Coordinated Colloids

ARG52748: IHC-P / Mouse

Wei-Peng Li et al.
ACS Nano. , (2017)

Obesity enhances carcinogen 7, 12-Dimethylbenz [a] anthracene -induced tumorigenesis in vitro and in vivo.

ARG52748: IHC-P / Mouse

Hsieh Chia-Chien et al.
Food Chem Toxicol., (2017)

Inflammasome & Pyroptosis

Inflammation

Ferroptosis

ARG30327

Microvascular Density Antibody Panel

Angiogenesis antibody; MVD Antibody; Microvascular Density Antibody