ARG30333
M1/M2/TAM Marker Antibody Panel
Publication19
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG10514 | anti-CD68 antibody [KP1] | Mouse mAb | Hu, Ms, Rat, Rb | FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB | 20 μg |
| ARG56509 | anti-iNOS antibody | Rabbit pAb | Hu, Ms, Rat, Mamm | ICC/IF, IHC-Fr, IHC-P, IP, WB | 50 μl |
| ARG55554 | anti-CD206 / MMR antibody [15-2] | Mouse mAb | Hu, Ms | CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB | 20 μg |
| ARG66630 | anti-CD163 antibody [SQab19148] | Rabbit mAb | Hu | IHC-P | 20 μl |
Overview
| Product Description | M1/M2/TAM Marker Antibody Panel is an all-in-one solution to make identification of macrophage subtypes easy and economic. This antibody panel comprises M1 macrophage markers CD68 and iNOS antibodies, and M2 macrophage markers CD163 and CD206 antibodies. Besides, all four antibodies are ideal for determining the tumor-associated macrophage (TAM) phenotype. All the antibodies in this panel have excellent IHC staining performance. Also, the different host species make double staining possible. Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
|---|---|
| Target Name | M1/M2/TAM Marker |
| Alternate Names | M1/M2/TAM Marker antibody; CD68 antibody; CD206 / MMR antibody; iNOS antibody; CD163 antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (14) Click the Picture to Zoom In
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ARG56509 anti-iNOS antibody IHC-P image
Immunohistochemistry: Rat Brain stained with ARG56509 anti-iNOS antibody at 1:100 dilution.
From Abrar Roshdy Abouelkeir et al. European Chemical Bulletin,(2023) doi: 10.31838/ecb/2023.12.1.470, Fig. 6.
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ARG56509 anti-iNOS antibody ICC/IF image (Customer's Feedback)
Immunofluorescence: RAW264.7 cells were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.1% Triton X-100 then blocked with 2% albumin for 60 min at RT. Cells were stained with ARG56509 anti-iNOS antibody (green) at 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG56509 anti-iNOS antibody WB image
Western blot: Rat Aortic stained with ARG56509 anti-iNOS antibody at 1:1000 dilution.
From Wahid Shah et al. Sci Rep. (2023), doi: 10.1038/s41598-023-43786-4, Fig. 2. C.
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
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ARG55554 anti-CD206 / MMR antibody [15-2] IHC-Fr image
Immunohistochemistry: Frozen section of Human tonsil tissue stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 1:25 dilution. The antibody stains endothelia of lymph vessels strongly.
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ARG56509 anti-iNOS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreatic ductal adenocarcinoma stained with ARG56509 anti-iNOS antibody.
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ARG56509 anti-iNOS antibody WB image
Western blot: Raw264.7 cells untreated or treated with LPS. 20 µg of cell lysates stained with ARG56509 anti-iNOS antibody at 1:400 dilution.
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ARG66630 anti-CD163 antibody [SQab19148] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human liver tissue stained with ARG66630 anti-CD163 antibody [SQab19148]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
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ARG10514 anti-CD68 antibody [KP1] WB image
Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].
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ARG55554 anti-CD206 / MMR antibody [15-2] FACS image
Flow Cytometry: Stimulated (GM-CSF + IL-4) human peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
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ARG10514 anti-CD68 antibody [KP1] FACS image
Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].
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ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG10514 anti-CD68 antibody [KP1] at 1:200 dilution.
Secondary antibody: Anti-Mouse IgG antibody (Rhodamine red).
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ARG55554 anti-CD206 / MMR antibody [15-2] FACS image
Flow Cytometry: Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed). Human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
Specific References
Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation
ARG10514: IHC-Fr / Mouse
Facile and rapid fabrication of a novel 3D-printable, visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair
ARG10514: IHC-P / Mouse
Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice
ARG56509: WB, IHC-P / Mouse
Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions
ARG56509: WB / Rat
Anti-Inflammatory Actions of G-Protein-Coupled Estrogen Receptor 1 (GPER) and Brain-Derived Estrogen Following Cerebral Ischemia in Ovariectomized Rats
ARG56509: WB, IHC-Fr / Rat
The Proinflammatory Role of Guanylate-Binding Protein 5 in Inflammatory Bowel Diseases
ARG10514: ICC-IF / Mouse
Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia
ARG56509: IHC-Fr / Mouse
Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats
ARG56509: IHC-P / Rat
Dual keratinocyte-attachment and anti-inflammatory coatings for soft tissue sealing around transmucosal oral implants
ARG56509: ICC/IF / Mouse
Glycolytic Reprogramming in Silica-Induced Lung Macrophages and Silicosis Reversed by Ac-SDKP Treatment
ARG56509: WB / Rat
Complement-mediated M2/M1 macrophage polarization may be involved in crescent formation in lupus nephritis
ARG10514: IHC-P / Human
Btk knockout attenuates the liver inflammation in STZ-induced diabetic mice by suppressing NLRP3 inflammasome activation.
ARG10514: IHC-P / Mouse
Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.
ARG10514: IHC-P / Human
TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.
ARG10514: IHC-P / Mouse
Baihe Wuyao decoction ameliorates CCl 4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects.
ARG56509: WB / Mouse
A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications.
ARG56509: IHC-P / Rat
Exosomes from high glucose-treated macrophages activate glomerular mesangial cells via TGF-β1/Smad3 pathway in vivo and in vitro.
ARG10514: IHC-P / Mouse
HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide.
ARG56509: IHC-P / Mouse
Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis
ARG56509: IHC-P / Rat
