ARG30333

M1 / M2 / TAM Marker Antibody Panel

Datasheet

Publication25

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Price (USD)

1 kit

410.00

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Datasheet
Publication (25)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG10514	anti-CD68 antibody [KP1]	Mouse mAb	Hu, Rat	FACS, ICC/IF, IHC-P, WB	20 μg
ARG56509	anti-iNOS antibody	Rabbit pAb	Hu, Ms, Rat, Mamm	ICC/IF, IHC-Fr, IHC-P, IP, WB	50 μl
ARG55554	anti-CD206 / MMR antibody [15-2]	Mouse mAb	Hu, Ms	CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB	20 μg
ARG66630	anti-CD163 antibody [SQab19148]	Rabbit mAb	Hu	IHC-P	20 μl

Overview

Product Description	M1 / M2 / TAM Marker Antibody Panel is an all-in-one solution to make identification of macrophage subtypes easy and economic. This antibody panel comprises M1 macrophage markers CD68 and iNOS antibodies, and M2 macrophage markers CD163 and CD206 antibodies. Besides, all four antibodies are ideal for determining the tumor-associated macrophage (TAM) phenotype. All the antibodies in this panel have excellent IHC staining performance. Also, the different host species make double staining possible. Related news: New antibody panels and duos for Tumor immune microenvironment< / font>< / a> Anti-SerpinB9 therapy, a new strategy for cancer therapy< / font>< / a> RIP1 activation and pathogenesis of NASH< / font>< / a>
Target Name	M1 / M2 / TAM Marker
Alternate Names	M1/M2/TAM Marker antibody; CD68 antibody; CD206 / MMR antibody; iNOS antibody; CD163 antibody

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Images (13) Click the Picture to Zoom In

ARG56509 anti-iNOS antibody IHC-P image

Immunohistochemistry: Rat Brain stained with ARG56509 anti-iNOS antibody at 1:100 dilution.

From Abrar Roshdy Abouelkeir et al. European Chemical Bulletin,(2023) doi: 10.31838/ecb/2023.12.1.470, Fig. 6.
ARG56509 anti-iNOS antibody ICC/IF image (Customer's Feedback)

Immunofluorescence: RAW264.7 cells were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.1% Triton X-100 then blocked with 2% albumin for 60 min at RT. Cells were stained with ARG56509 anti-iNOS antibody (green) at 4°C. DAPI (blue) was used as the nuclear counter stain.
ARG56509 anti-iNOS antibody WB image

Western blot: Rat Aortic stained with ARG56509 anti-iNOS antibody at 1:1000 dilution.

From Wahid Shah et al. Sci Rep. (2023), doi: 10.1038/s41598-023-43786-4, Fig. 2. C.
ARG10514 anti-CD68 antibody [KP1] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
ARG55554 anti-CD206 / MMR antibody [15-2] IHC-Fr image

Immunohistochemistry: Frozen section of Human tonsil tissue stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 1:25 dilution. The antibody stains endothelia of lymph vessels strongly.
ARG56509 anti-iNOS antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human pancreatic ductal adenocarcinoma stained with ARG56509 anti-iNOS antibody.
ARG56509 anti-iNOS antibody WB image

Western blot: Raw264.7 cells untreated or treated with LPS. 20 µg of cell lysates stained with ARG56509 anti-iNOS antibody at 1:400 dilution.
ARG66630 anti-CD163 antibody [SQab19148] IHC-P image

Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human liver tissue stained with ARG66630 anti-CD163 antibody [SQab19148]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
ARG10514 anti-CD68 antibody [KP1] WB image

Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].
ARG55554 anti-CD206 / MMR antibody [15-2] FACS image

Flow Cytometry: Stimulated (GM-CSF + IL-4) human peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
ARG10514 anti-CD68 antibody [KP1] FACS image

Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).
ARG10514 anti-CD68 antibody [KP1] IHC-P image

Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].
ARG55554 anti-CD206 / MMR antibody [15-2] FACS image

Flow Cytometry: Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed). Human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.

Specific References

Identification and experimental verification of immune-related hub genes in intervertebral disc degeneration

ARG10514: ICC/IF / Human

Zeling Huang et al.
Heliyon., (2024)

Cardamonin intervenes in myocardial hypertrophy progression by regulating Usp18

ARG10514: IHC-P / Mouse

Zhenyu Feng et al.
Phytomedicine., (2024)

Effect of macrophage-to-myofibroblast transition on silicosis

ARG56509: IHC-P,WB / Rat

Fei Geng et al.
Anim Models Exp Med., (2024)

Aquaporin-9 Aggravates Lipopolysaccharides Induced Acute Lung Injury Via Facilitating M1-Like Macrophage Polarization

ARG10514: IHC-P / Mouse

Jiajia Wan et al.
preprint, (2024)

Facile and rapid fabrication of a novel 3D-printable, visible light-crosslinkable and bioactive polythiourethane for large-to-massive rotator cuff tendon repair

ARG10514: IHC-P / Mouse

Xu Zhang et al.
Bioact Mater., (2024)

Banxia-Houpu decoction inhibits iron overload and chronic intermittent hypoxia-induced neuroinflammation in mice

ARG56509: WB, IHC-P / Mouse

Xin-Yue Yang et al.
J Ethnopharmacol., (2024)

Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice

ARG56509: IHC-P / Mouse

Zhenyu Feng et al.
Heliyon, (2024)

Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation

ARG10514: IHC-Fr / Mouse

Qiu-Yue Lin et al.
BBA - Molecular Basis of Disease, (2024)

Polarização dos macrófagos e sua associação com fatores prognósticos em casos de neoplasias colorretais, considerando a presença e ausência de mutações nas metaloproteinases

ARG66630: IHC-P / Human

Brambilla, Eduardo et al.
Doutorado em Ciências da Saúde, (2024)

Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions

ARG56509: WB / Rat

Wahid Shah et al.
Sci Rep., (2023)

Anti-Inflammatory Actions of G-Protein-Coupled Estrogen Receptor 1 (GPER) and Brain-Derived Estrogen Following Cerebral Ischemia in Ovariectomized Rats

ARG56509: WB, IHC-Fr / Rat

Jing Xu et al.
Biology (Basel)., (2023)

Dual keratinocyte-attachment and anti-inflammatory coatings for soft tissue sealing around transmucosal oral implants

ARG56509: ICC/IF / Mouse

Sunil Kumar Boda. et al.
Biomater Sci., (2022)

The Proinflammatory Role of Guanylate-Binding Protein 5 in Inflammatory Bowel Diseases

ARG10514: ICC/IF / Mouse

Yichen Li et al.
Front Microbiol ., (2022)

Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats

ARG56509: IHC-P / Rat

Man Wang et al.
BMC Complement Med Ther, (2022)

Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia

ARG56509: IHC-Fr / Mouse

Miao Tang et al.
Pharm Biol., (2022)

Glycolytic Reprogramming in Silica-Induced Lung Macrophages and Silicosis Reversed by Ac-SDKP Treatment

ARG56509: WB / Rat

Na Mao et al.
Int J Mol Sci., (2021)

Btk knockout attenuates the liver inflammation in STZ-induced diabetic mice by suppressing NLRP3 inflammasome activation.

ARG10514: IHC-P / Mouse

Yuanyuan Li et al.
Biochem Biophys Res Commun., (2021)

Enhanced Bruton's tyrosine kinase activity in the kidney of patients with IgA nephropathy.

ARG10514: IHC-P / Human

Jie Wei et al.
Int Urol Nephrol., (2021)

Complement-mediated M2/M1 macrophage polarization may be involved in crescent formation in lupus nephritis

ARG10514: IHC-P / Human

Juan Tao et al.
Int Immunopharmacol., (2021)

TAB1 regulates glycolysis and activation of macrophages in diabetic nephropathy.

ARG10514: IHC-P / Mouse

Hanxu Zeng et al.
Inflamm Res., (2020)

Baihe Wuyao decoction ameliorates CCl 4-induced chronic liver injury and liver fibrosis in mice through blocking TGF-β1/Smad2/3 signaling, anti-inflammation and anti-oxidation effects.

ARG56509: WB / Mouse

Yajing Chen et al.
J Ethnopharmacol., (2020)

A free-standing multilayer film as a novel delivery carrier of platelet lysates for potential wound-dressing applications.

ARG56509: IHC-P / Rat

Tang Qian et al.
Biomaterials., (2020)

Exosomes from high glucose-treated macrophages activate glomerular mesangial cells via TGF-β1/Smad3 pathway in vivo and in vitro.

ARG10514: IHC-P / Mouse

Zhu Qi-jin et al.
FASEB J., (2019)

HYCO-3, a dual CO-releaser/Nrf2 activator, reduces tissue inflammation in mice challenged with lipopolysaccharide.

ARG56509: IHC-P / Mouse

Motterlini Roberto et al.
Redox Biol., (2018)

Distribution and proportion of M1/M2 macrophages in periodontal tissues in rats with and without periodontitis