ARG81420
Human TIMP3 ELISA Kit
Human TIMP3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81420-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81420-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81420-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81420-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81420-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81420-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81420-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81420-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81420-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81420-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81420-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81420 Human TIMP3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIMP3 in serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TIMP3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7% Inter-Assay CV: 8.3% |
| Alternate Names | HSMRK222; K222TA2; TIMP-3; Protein MIG-5; K222; SFD; Metalloproteinase inhibitor 3; Tissue inhibitor of metalloproteinases 3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TIMP3 |
| Gene Full Name | TIMP metallopeptidase inhibitor 3 |
| Background | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008] |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. [UniProt] |
| Highlight | Related products: TIMP3 antibodies; TIMP3 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81420 Human TIMP3 ELISA Kit standard curve image
ARG81420 Human TIMP3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81420 Human TIMP3 ELISA Kit User manual |
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