ARG80187
Human TIMP1 ELISA Kit
Human TIMP1 ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80187-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80187-002 | Standard (Lyophilized) | 2 X 1 ng/vial | 4°C |
| ARG80187-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80187-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80187-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80187-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80187-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80187-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80187-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80187-010 | STOP solution | 12ml | 4°C |
| ARG80187-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80187 Human TIMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIMP1 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human MMP-1, MMP-3, MMP-2, TIMP-2；mouse TIMP-1 |
| Target Name | TIMP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 16 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | Erythroid-potentiating activity; TIMP; Collagenase inhibitor; Fibroblast collagenase inhibitor; TIMP-1; EPO; CLGI; Tissue inhibitor of metalloproteinases 1; HCI; Metalloproteinase inhibitor 1; EPA |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TIMP1 |
| Gene Full Name | TIMP metallopeptidase inhibitor 1 |
| Background | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq] |
| Highlight | Related products: TIMP1 antibodies; TIMP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
| PTM | The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG80187 Human TIMP1 ELISA Kit standard curve image
ARG80187 Human TIMP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80187 Human TIMP1 ELISA Kit User's manual |
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