ARG81989
Human TIM3 ELISA Kit
Human TIM3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81989-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81989-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81989-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81989-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81989-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81989-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81989-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81989-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81989-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81989-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81989-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81989 Human TIM3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TIM3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | TIM3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4%; Inter-Assay CV: 6.1% |
| Alternate Names | KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8TDQ0 Human Hepatitis A virus cellular receptor 2 |
|---|---|
| Gene Symbol | HAVCR2 |
| Gene Full Name | hepatitis A virus cellular receptor 2 |
| Background | The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011] |
| Function | Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. [UniProt] |
| Highlight | Related products: TIM3 antibodies; TIM3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | O-glycosylated with core 1 or possibly core 8 glycans. Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81989 Human TIM3 ELISA Kit standard curve image
ARG81989 Human TIM3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81989 Human TIM3 ELISA Kit User manual |
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