ARG81526
Human SPARC ELISA Kit
Human SPARC ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81526-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81526-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81526-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81526-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81526-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81526-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81526-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81526-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81526-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81526-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81526-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81526 Human SPARC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SPARC in serum, human milk and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | SPARC |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, human milk and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5% Inter-Assay CV: 7.5% |
| Alternate Names | ON; BM-40; Basement-membrane protein 40; SPARC; Osteonectin; Secreted protein acidic and rich in cysteine |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SPARC |
| Gene Full Name | secreted protein, acidic, cysteine-rich (osteonectin) |
| Background | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015] |
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. [UniProt] |
| Highlight | Related products: SPARC antibodies; SPARC ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81526 Human SPARC ELISA Kit standard curve image
ARG81526 Human SPARC ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81526 Human SPARC ELISA Kit User's manual |
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