ARG80173
Human Perforin ELISA Kit
Human Perforin ELISA Kit for ELISA and Human
Immune System kit
Publication6
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80173-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80173-002 | Standard (Lyophilized) | 2 X 2.5 ng/vial | 4°C |
| ARG80173-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80173-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80173-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80173-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80173-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80173-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80173-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80173-010 | STOP solution | 12ml | 4°C |
| ARG80173-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80173 Human Perforin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Perforin in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-1 beta, IL-2, IL-3, IFN- gamma, TNF alpha, Fas, Fas-L, TRAIL |
| Target Name | Perforin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 20 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 39 - 2500 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRF1 |
| Gene Full Name | perforin 1 (pore forming protein) |
| Background | Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] |
| Function | Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt] |
| Highlight | Related products: Perforin antibodies; Perforin ELISA Kits; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
| PTM | N-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG80173 Human Perforin ELISA Kit standard curve image
ARG80173 Human Perforin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Redirecting natural killer cells to potentiate adoptive immunotherapy in solid tumors through stabilized Y-type bispecific aptamer
ELISA / Human / Cell culture supernatants
Acute lymphoblastic leukemia-derived exosome inhibits cytotoxicity of natural killer cells by TGF-β signaling pathway
ELISA / Human / Cell culture supernatants
B7-H3 inhibits the IFN-γ-dependent cytotoxicity of Vγ9Vδ2 T cells against colon cancer cells.
ELISA / Human / Cell culture supernatant
Tim-3 suppresses the killing effect of Vγ9Vδ2 T cells on colon cancer cells by reducing perforin and granzyme B expression.
ELISA / Human
The Effect of Tea Catechins on Natural Killer Cell Activity in the Elderly: A Pilot Study.
ELISA / Human
Increase of Tumor Infiltrating γδ T-cells in Pancreatic Ductal Adenocarcinoma Through Remodeling of the Extracellular Matrix by a Hyaluronan Synthesis Suppressor, 4-Methylumbelliferone.
ELISA / Human
| Title | Download Link |
|---|---|
| ARG80173 Human Perforin ELISA Kit User's manual |
 Download
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