ARG82774

Human Peptide YY ELISA Kit

Human Peptide YY ELISA Kit for ELISA and Human

Overview

Product Description ARG82774 Human Peptide YY ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Peptide YY (3-36) and Human Peptide YY (1-36) in serum and plasma.
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross Reactivity:
Human Peptide YY (3-36): 100%
Human Peptide YY: 100%
Mouse, Rat, Pig and Dog Peptide YY: 26%
Mouse, Rat, Pig and Dog Peptide YY (3-36): 10%
Pig Neuropeptide Y: 11%
Human Pancreatic Polypeptide, Human Insulin, Human Glucagon, Human Amylin Amide and Substance P: 0%
Note: The standard in the kit is Human Peptide YY (3-36) peptide, and this kit also detects Human Peptide YY (1-36).
Target Name Peptide YY
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.079 ng/ml
Detection Range Linear range: 0.079 - 0.88 ng/ml
Sample Type Serum and plasma
Standard Range 0.01 - 100 ng/ml
Sample Volume 50 µl
Alternate Names Peptide tyrosine tyrosine; PYY; PYY-I; PYY1; Peptide YY; PYY-II; 3-36

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5697 Human PYY

Swiss-port # P10082 Human Peptide YY

Gene Symbol PYY
Gene Full Name peptide YY
Background This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded preproprotein is proteolytically processed to generate two alternative peptide products that differ in length by three amino acids. These peptides, secreted by endocrine cells in the gut, exhibit different binding affinities for each of the neuropeptide Y receptors. Binding of the encoded peptides to these receptors mediates regulation of pancreatic secretion, gut mobility and energy homeostasis. Rare variations in this gene could increase susceptibility to obesity and elevated serum levels of the encoded peptides may be associated with anorexia nervosa. [provided by RefSeq, Feb 2016]
Function This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. [UniProt]
Cellular Localization Secreted. [UniProt]
PTM The peptide YY form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro) to generate peptide YY(3-36). [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82774 Human Peptide YY ELISA Kit standard curve image

    ARG82774 Human Peptide YY ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82774 Human Peptide YY ELISA Kit User manual download icon Download