ARG80166
Human PDGF BB ELISA Kit
Human PDGF BB ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80166-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80166-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80166-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80166-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80166-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80166-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80166-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80166-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80166-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80166-010 | STOP solution | 12ml | 4°C |
| ARG80166-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80166 Human PDGF BB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PDGF BB in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human PDGF R alpha |
| Target Name | PDGF BB |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01127 Human Platelet-derived growth factor subunit B |
|---|---|
| Gene Symbol | PDGFB |
| Gene Full Name | platelet-derived growth factor beta polypeptide |
| Background | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Highlight | Related products: PDGF BB antibodies; PDGF BB ELISA Kits; Related news: Vascular development is regulated by FGF-dependent metabolic control The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80166 Human PDGF BB ELISA Kit standard curve image
ARG80166 Human PDGF BB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80166 Human PDGF-BB ELISA Kit User's manual |
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