ARG81508
Human Nesfatin 1 ELISA Kit
Human Nesfatin 1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81508-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81508-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81508-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81508-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81508-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81508-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81508-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81508-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81508-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81508-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81508-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81508 Human Nesfatin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Nesfatin 1 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Nesfatin 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 6.6% |
| Alternate Names | HEL-S-109; NEFA; Nucleobindin-2; Gastric cancer antigen Zg4; Prepronesfatin; DNA-binding protein NEFA |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NUCB2 |
| Gene Full Name | nucleobindin 2 |
| Background | This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011] |
| Function | Calcium-binding protein. May have a role in calcium homeostasis. Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity). [UniProt] |
| Highlight | Related products: Nesfatin antibodies; Nesfatin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81508 Human Nesfatin 1 ELISA Kit standard curve image
ARG81508 Human Nesfatin 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81508 Human Nesfatin 1 ELISA Kit User's manual |
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