ARG81414

Human MMP7 (total) ELISA Kit

Human MMP7 (total) ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81414-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81414-002 Standard 2 X 10 ng/vial 4°C
ARG81414-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81414-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81414-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81414-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81414-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81414-008 25X Wash buffer 20 ml 4°C
ARG81414-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81414-010 STOP solution 10 ml (Ready to use) 4°C
ARG81414-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81414 Human MMP7 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human total MMP7 in serum, plasma (heparin), saliva, urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MMP7
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin), saliva, urine and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 6.3%
Alternate Names MMP-7; Uterine metalloproteinase; Matrix metalloproteinase-7; EC 3.4.24.23; Matrilysin; Matrin; Pump-1 protease; PUMP-1; MPSL1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4316 Human MMP7

Swiss-port # P09237 Human Matrilysin

Gene Symbol MMP7
Gene Full Name matrix metallopeptidase 7
Background MMP7 is a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. [provided by RefSeq, Jan 2016]
Function MMP7 degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. [UniProt]
Highlight Related products:
MMP7 antibodies; MMP7 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG81414 Human MMP7 ELISA Kit standard curve image

    ARG81414 Human MMP7 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81414 Human MMP7 (total) ELISA Kit User's manual download icon Download