ARG82314
Human MMP13 ELISA Kit
Human MMP13 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82314-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82314-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82314-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82314-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82314-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82314-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82314-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82314-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82314-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82314-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82314-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82314 Human MMP13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MMP13 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MMP13 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.078 ng/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 0.156 - 10 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7% Inter-Assay CV: 7.9% |
| Alternate Names | CLG3; EC 3.4.24.-; MANDP1; MMP-13; Collagenase 3; Matrix metalloproteinase-13 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP13 |
| Gene Full Name | matrix metallopeptidase 13 |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| Function | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. [UniProt] |
| Highlight | Related products: MMP13 antibodies; MMP13 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The proenzyme is activated by removal of the propeptide; this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro). N-glycosylated. Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG82314 Human MMP13 ELISA Kit standard curve image
ARG82314 Human MMP13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82314 Human MMP13 ELISA Kit User manual |
 Download
|
