ARG81488
Human MMP12 ELISA Kit
Human MMP12 ELISA Kit for ELISA and Human
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81488-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81488-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81488-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81488-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81488-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81488-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81488-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81488-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81488-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81488-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81488-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81488 Human MMP12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MMP12 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MMP12 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 6.2% |
| Alternate Names | ME; EC 3.4.24.65; Macrophage metalloelastase; MMP-12; hME; MME; HME; Matrix metalloproteinase-12; Macrophage elastase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP12 |
| Gene Full Name | matrix metallopeptidase 12 |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| Function | May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. [UniProt] |
| Highlight | Related products: MMP12 antibodies; MMP12 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81488 Human MMP12 ELISA Kit standard curve image
ARG81488 Human MMP12 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Adipose-derived stem cells enriched with therapeutic mRNA TGF-β3 and IL-10 synergistically promote scar-less wound healing in preclinical models
ELISA / Human / Culture supernatant
| Title | Download Link |
|---|---|
| ARG81488 Human MMP12 ELISA Kit User's manual |
 Download
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