ARG80167
Human MIP1 alpha / CCL3 ELISA Kit
Human MIP1 alpha / CCL3 ELISA Kit for ELISA and Human
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80167-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80167-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80167-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80167-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80167-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80167-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80167-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80167-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80167-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80167-010 | STOP solution | 12ml | 4°C |
| ARG80167-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80167 Human MIP1 alpha / CCL3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MIP1 alpha / CCL3 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human MIP-1β,MIP-1δ,MIP-3α,MIP-3β |
| Target Name | CCL3 / MIP1 alpha |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | MIP-1-alpha; SIS-beta; PAT 464.1; Tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; Small-inducible cytokine A3; C-C motif chemokine 3; LD78-alpha; 4-69; Macrophage inflammatory protein 1-alpha; MIP1A; SCYA3; G0S19-1; LD78ALPHA |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL3 |
| Gene Full Name | chemokine (C-C motif) ligand 3 |
| Background | MIP1 alpha belongs to the chemokine beta family. In vitro MIP1 alpha stimulates H2O2 production in human neutrophils. MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor 1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated. |
| Highlight | Related products: CCL3 antibodies; CCL3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
| PTM | N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. |
Images (1) Click the Picture to Zoom In
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ARG80167 Human MIP1 alpha / CCL3 ELISA Kit standard curve image
ARG80167 Human MIP1 alpha / CCL3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80167 Human MIP1 alpha / CCL3 ELISA Kit User's manual |
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