ARG82623
Human MCP3 ELISA Kit
Human MCP3 ELISA Kit for ELISA and Human
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82623-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82623-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82623-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82623-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82623-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82623-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82623-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82623-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82623-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82623-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82623-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82623 Human MCP3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MCP3 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | MCP3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.0% Inter-Assay CV: 7.1% |
| Alternate Names | MCP3; NC28; Monocyte chemotactic protein 3; MARC; Small-inducible cytokine A7; FIC; C-C motif chemokine 7; Monocyte chemoattractant protein 3; MCP-3; SCYA7; SCYA6 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL7 |
| Gene Full Name | chemokine (C-C motif) ligand 7 |
| Background | This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008] |
| Function | Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: MCP3 antibodies; MCP3 ELISA Kits; MCP3 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82623 Human MCP3 ELISA Kit standard curve image
ARG82623 Human MCP3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
The Synergistic Inhibition of Coronavirus Replication and Induced Cytokine Production by Ciclesonide and the Tylophorine-Based Compound Dbq33b
ELISA / Human / Culture supernatant
| Title | Download Link |
|---|---|
| ARG82623 Human MCP3 ELISA Kit User manual |
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