ARG81952
Human LDL receptor ELISA Kit
Human LDL receptor ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81952-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81952-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81952-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81952-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81952-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81952-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81952-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81952-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81952-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81952-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81952-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81952 Human LDL receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LDL receptor in serum, plasma (heparin), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | LDL Receptor |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin), urine and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1%; Inter-Assay CV: 6.6% |
| Alternate Names | FH; LDLCQ2; Low-density lipoprotein receptor; LDL receptor; FHC |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LDLR |
| Gene Full Name | low density lipoprotein receptor |
| Background | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010] |
| Function | Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. [UniProt] |
| Highlight | Related products: LDL Receptor antibodies; LDL Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. Ubiquitinated by MYLIP leading to degradation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81952 Human LDL receptor ELISA Kit standard curve image
ARG81952 Human LDL receptor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81952 Human LDL receptor ELISA Kit User's manual |
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