ARG80175
Human IgG4 ELISA Kit
Human IgG4 ELISA Kit for ELISA and Human
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80175-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80175-002 | Standard (Lyophilized) | 3 X 0.7 μg/vial | 4°C |
| ARG80175-003 | Standard diluent buffer | 40 ml | 4°C |
| ARG80175-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80175-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80175-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80175-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80175-010 | STOP solution | 12ml | 4°C |
| ARG80175-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80175 Human IgG4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IgG4 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IgG1, IgG2, IgG3 |
| Target Name | IgG4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 ng/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 15.6 - 1000 ng/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | Immunoglobulin G4 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | IgG4 is present in very low levels in children younger than 10 years of age, so IgG4 deficiencies are not usually diagnosed before age 10. IgG4 may be undetectable in the serum of many “normal” adult individuals, and therefore low IgG4 alone is insufficient evidence of an antibody deficiency disorder requiring Ig replacement. IgG4–related systemic disease (IgG4-RSD), also known as hyper-IgG4 disease and IgG4-related disease is a disease in which inflammatory cells cause fibrosis, the deposition of connective tissue, in one or more organs. The disease is so named because the antibody subtype IgG4 can be detected on tissue samples and often at elevated levels in the bloodstream. The association with IgG4 is a relatively recent finding, and the condition has been described under numerous other names in the past. Diseases such asautoimmune pancreatitis, retroperitoneal fibrosis, mediastinal fibrosis, Riedel's thyroiditis, Mikulicz's syndrome, Kuttner's tumor and inflammatory pseudotumor are now regarded as forms of IgG4-RSD. |
|---|---|
| Highlight | Related products: IgG4 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
-
ARG80175 Human IgG4 ELISA Kit standard curve image
ARG80175 Human IgG4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80175 Human IgG4 ELISA Kit User's manual |
 Download
|
