ARG70065
Human IL21 recombinant protein (Active) (His-tagged, C-ter)
Human IL21 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL21 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL21 |
| Species | Human |
| A.A. Sequence | Gln32 - Ser162 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to enhance IFN gamma secretion in NK-92 cells. The ED50 for this effect is <10 ng/mL. |
| Alternate Names | Za11; IL-21; CVID11; Interleukin-21 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL21 |
|---|---|
| Gene Full Name | interleukin 21 |
| Background | This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| Function | Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70065 Human IL21 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70065 Human IL21 recombinant protein (Active) (His-tagged, C-ter).
