ARG80101

Human IL1 beta ELISA Kit

Human IL1 beta ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
publication_link Publication7

Component

Cat No Component Name Package Temp
ARG80101-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80101-002 Standard (Lyophilized) 3 X 0.6 ng/vial 4°C
ARG80101-003 Standard diluent buffer 20 ml 4°C
ARG80101-004 Antibody conjugate concentrate 400 µl 4°C
ARG80101-005 Antibody diluent buffer 16 ml 4°C
ARG80101-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80101-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80101-008 20X Wash buffer 50 ml 4°C
ARG80101-009 TMB substrate 12ml 4°C (Protect from light)
ARG80101-010 STOP solution 12ml 4°C
ARG80101-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80101 Human IL1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL1 beta in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-1 alpha;mouse IL-1 alpha, IL-1 beta;rat IL-1 alpha;porcine IL-1 alpha, IL-1 beta
Target Name IL1 beta
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 4 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision CV: less than10%
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3553 Human IL1B

Swiss-port # P01584 Human Interleukin-1 beta

Gene Symbol IL1B
Gene Full Name interleukin 1, beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
Highlight Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Activation of IL-1 beta and IL-18 by Canonical Inflammasome Pathway
LKB1 deficiency in T cells promotes gut tumors
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Images (1) Click the Picture to Zoom In

  • ARG80101 Human IL1 beta ELISA Kit standard curve image

    ARG80101 Human IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis

ELISA / Human / Culture supernatant

Wei-Ching Huang et al.
Respir Res.,  (2023)

publication_link

 

hr_line

IL-17C neutralization protects the kidney against acute injury and chronic injury

ELISA / Human / Cell culture supernatants

Fangfei Zhang et al.
EBioMedicine.,  (2023)

publication_link

 

hr_line

Macrophage secretory IL-1β promotes docetaxel resistance in head and neck squamous carcinoma via SOD2/CAT-ICAM1 signaling

ELISA / Human / Cell culture supernatant

Ching-Yun Hsieh et al.
JCI Insight,  (2022)

publication_link

 

hr_line

Role of P2X7R in eosinophilic and non‑eosinophilic chronic rhinosinusitis with nasal polyps.

ELISA / Human / Cell culture supernatant

Yan Wang et al.
Mol Med Rep.,  (2021)

publication_link

 

hr_line

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ELISA / Human / Cell culture medium

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

hr_line

Serum and peritoneal fluid levels of interleukin-6 and interleukin-37 as biomarkers for endometriosis.

ELISA / Human

Jiang Jianfa et al.
Gynecol Endocrinol.,  (2019)

publication_link

 

hr_line

Role of Different Salmonella enterica Serotypes in THP-1 Macrophage-Derived Foam Cell Autophagy and Inflammatory Response.

ELISA / Human

Wong Min-Yi et al.
Sains Malaysiana.,  (2019)

publication_link

 

hr_line
Title Download Link
ARG80101 Human IL1 beta ELISA Kit User manual download icon Download