ARG70042
Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter)
Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL1 alpha recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL1 alpha |
| Species | Human |
| A.A. Sequence | Ser113 - Ala271 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is <10 pg/mL. The specific activity of recombinant human IL-1 alpha is approximately >1 x10^8 IU/ mg. |
| Alternate Names | IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL1A |
|---|---|
| Gene Full Name | interleukin 1, alpha |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008] |
| Function | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt] |
| Cellular Localization | Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. [UniProt] |
| Highlight | Related products: IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines |
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ARG70042 Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70042 Human IL1 alpha recombinant protein (Active) (His-tagged, C-ter).
