ARG83352

Human IL1 alpha ELISA Kit

Human IL1 alpha ELISA Kit for ELISA and Human

Immune System kit; Metabolism kit

Component

Cat No Component Name Package Temp
ARG83352-01 Antibody Coated Microplate 8 X 12 strips 4°C
ARG83352-02 Standard (Lyophilized) 3 vials 4°C
ARG83352-03 100X Antibody Conjugate Concentrate 120 μl ≤ -20°C
ARG83352-04 1000X HRP-Streptavidin solution 15 μl 4°C
ARG83352-05 Standard / Sample Diluent Buffer 20 ml 4°C
ARG83352-06 Antibody Diluent Buffer 35 ml 4°C
ARG83352-07 10X Wash Buffer 50 ml 4°C
ARG83352-08 TMB substrate 12 ml 4°C (protect from light)
ARG83352-09 STOP solution 12 ml 4°C
ARG83352-10 Plate sealer 3 adhesive strips Room temperature

Overview

Product Description ARG83352 Human IL1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL1 alpha in Serum, Plasma and Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-1ra, IL-1 beta, IL-1 RI, IL-1 RII;mouse IL-1 alpha;porcine IL-1 alpha;rat IL-1 alpha
Target Name IL1 alpha
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 7.8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 pg/ml
Sample Volume 50 µl
Precision CV: less than10%
Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Application Instructions

Assay Time 4.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3552 Human IL1A

Swiss-port # P01583 Human Interleukin-1 alpha

Gene Symbol IL1A
Gene Full Name interleukin 1, alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq]
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Research Area Immune System kit; Metabolism kit

Images (1) Click the Picture to Zoom In

  • ARG83352 Human IL1 alpha ELISA Kit standard curve image

    ARG83352 Human IL1 alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG83352 Human IL1 alpha ELISA Kit User manual download icon Download