ARG80171
Human Granzyme B ELISA Kit
Human Granzyme B ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit
Publication3
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80171-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80171-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80171-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80171-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80171-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80171-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80171-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80171-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80171-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80171-010 | STOP solution | 12ml | 4°C |
| ARG80171-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80171 Human Granzyme B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granzyme B in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with other soluble cytokine |
| Target Name | Granzyme B |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GZMB |
| Gene Full Name | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
| Background | Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016] |
| Function | Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt] |
| Highlight | Related products: Granzyme B antibodies; Granzyme B ELISA Kits; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA Anti-SerpinB9 therapy, a new strategy for cancer therapy New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG80171 Human Granzyme B ELISA Kit standard curve image
ARG80171 Human Granzyme B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
B7-H3 inhibits the IFN-γ-dependent cytotoxicity of Vγ9Vδ2 T cells against colon cancer cells.
ELISA / Human / Cell culture supernatant
Tim-3 suppresses the killing effect of Vγ9Vδ2 T cells on colon cancer cells by reducing perforin and granzyme B expression.
ELISA / Human
The Effect of Tea Catechins on Natural Killer Cell Activity in the Elderly: A Pilot Study.
ELISA / Human
| Title | Download Link |
|---|---|
| ARG80171 Human Granzyme B ELISA Kit User's manual |
 Download
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