ARG80185
Human GRO beta / CXCL2 ELISA Kit
Human GRO beta / CXCL2 ELISA Kit for ELISA and Human
Immune System kit
Publication2
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80185-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80185-002 | Standard (Lyophilized) | 3 X 1 ng/vial | 4°C |
| ARG80185-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80185-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80185-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80185-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80185-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80185-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80185-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80185-010 | STOP solution | 12ml | 4°C |
| ARG80185-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80185 Human GRO beta / CXCL2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GRO beta / CXCL2 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human CXCL1, CXCL3, CXCL4, IL-2,3,4,5,6,7,9,10,12, IFN gamma and TNF alpha. |
| Target Name | CXCL2 / MIP2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 1.56 - 100 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: <10%; Inter-Assay CV: <10% |
| Alternate Names | Gro-beta; SCYB2; HSF; CINC-2a; GROb; MGSA-b; SB-251353; MIP2A; MIP2; Hematopoietic synergistic factor; 5-73; C-X-C motif chemokine 2; MIP-2a; GRO2; Macrophage inflammatory protein 2-alpha; GRO-beta-T; Growth-regulated protein beta; MIP2-alpha |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CXCL2 |
| Gene Full Name | chemokine (C-X-C motif) ligand 2 |
| Background | Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. |
| Highlight | Related products: GRO beta antibodies; GRO beta ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
| PTM | The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. |
Images (1) Click the Picture to Zoom In
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ARG80185 Human GRO beta / CXCL2 ELISA Kit standard curve image
ARG80185 Human GRO beta / CXCL2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors.
ELISA / Human / Cell culture supernatant
Mesenchymal stromal cells release CXCL1/2/8 and induce chemoresistance and macrophage polarization.
ELISA / Human
| Title | Download Link |
|---|---|
| ARG80185 Human GRO beta / CXCL2 ELISA Kit User's manual |
 Download
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