ARG83044
Human EGFR ELISA Kit
Human EGFR ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83044 Human EGFR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human EGFR in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Not cross-reacts with: Human EGF, ErbB3, ErbB2, ErbB4. Mouse EGF. |
| Target Name | EGFR |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Epidermal growth factor receptor |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | EGFR, Epidermal Growth Factor Receptor, ERBB1, ERRP, ERBB, Receptor Tyrosine Protein Kinase ErbB1, Erb B2 Receptor Tyrosine Kinase 1, Proto Oncogene C ErbB 1, EC 2.7.10.1, HER1, Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V Erb B) Oncogene Homolog), Erythroblastic Leukemia Viral (V Erb B) Oncogene Homolog (Avian), Avian Erythroblastic Leukemia Viral (V Erb B) Oncogene Homolog, Epidermal Growth Factor Receptor Tyrosine Kinase Domain, Cell Proliferation Inducing Protein 61, Cell Growth Inhibiting Protein 40, EGFR VIII, EC 2.7.10, NISBD2, PIG61, MENA |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EGFR |
| Gene Full Name | Epidermal Growth Factor Receptor |
| Background | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020] |
| Function | Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. [Uniprot] |
| Cellular Localization | Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Nucleus, Secreted. [UniProt] |
| Highlight | Related products: EGFR antibodies; EGFR ELISA Kits; EGFR Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83044 Human EGFR ELISA Kit standard curve image
ARG83044 Human EGFR ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83044 Human EGFR ELISA Kit User manual |
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