ARG81623
Human DAND5 ELISA Kit
Human DAND5 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81623-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81623-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81623-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81623-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81623-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81623-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81623-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81623-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81623-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81623-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81623-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81623 Human DAND5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DAND5 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | DAND5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 6.9% |
| Alternate Names | CRL2; Cerberus-like protein 2; GREM3; SP1; Gremlin-3; DANTE; CKTSF1B3; CER2; Cerl-2; COCO; CERL2; DAN domain family member 5; Cysteine knot superfamily 1, BMP antagonist 3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DAND5 |
| Gene Full Name | DAN domain family member 5, BMP antagonist |
| Background | This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry. [provided by RefSeq, Jul 2008] |
| Function | Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation (By similarity). [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81623 Human DAND5 ELISA Kit standard curve image
ARG81623 Human DAND5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81623 Human DAND5 ELISA Kit User's manual |
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